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Construction strategies for developing expression vectors for recombinant monoclonal antibody production in CHO cells

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Abstract

Recent years have seen the use of recombinant proteins in the treatment of different diseases. Among them, monoclonal antibodies (mAbs) are currently the fastest growing class of bio-therapeutic recombinant proteins. Chinese hamster ovary (CHO) cells are the most commonly used host cells for production of these recombinant mAbs. Expression vectors determine the expression level and quality of recombinant mAbs. Currently, few construction strategies for recombinant mAbs expression vectors in CHO cells have been developed, including monocistronic vector, multiple-promoter expression vector, and tricistronic vector mediated by internal ribosome entry site (IRES) or Furin-2A element. Among them, Furin-2A-mediated vector is an effective approach due to advantages of high “self-cleavage” efficiency, and equal expression of light and heavy chains from a single open reading frame. Here, we have reviewed the progress in development of different strategies for constructing recombinant mAb expression vectors in CHO cells and its potential advantages and disadvantages.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 81673337), and Program for Innovative Research Team (in Science and Technology) in University of Henan Province (No. 18IRTSTHN027).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Jinsui Road, Xinxiang, 453003, Henan, People’s Republic of China

    Yan-mei Li, Zheng-wei Tian, Dan-hua Xu, Xiao-yin Wang & Tian-yun Wang

  2. International Joint Research Laboratory for Recombiant Pharmaceutical Protein Expression System of Henan, Xinxiang Medical University, Jinsui Road, Xinxiang, 453003, Henan, People’s Republic of China

    Yan-mei Li, Zheng-wei Tian, Dan-hua Xu, Xiao-yin Wang & Tian-yun Wang

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  1. Yan-mei Li
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  2. Zheng-wei Tian
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Correspondence to Tian-yun Wang.

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Li, Ym., Tian, Zw., Xu, Dh. et al. Construction strategies for developing expression vectors for recombinant monoclonal antibody production in CHO cells. Mol Biol Rep 45, 2907–2912 (2018). https://doi.org/10.1007/s11033-018-4351-0

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