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Association of resistin polymorphisms with resistin levels and lipid profile in children

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Abstract

Previous research has found a correlation between resistin and lipid level variations. Polymorphisms in the resistin gene (RETN) could be involved in this relationship, but the results of the different studies are contradictory. The aim of this study was to examine the association between resistin and lipid levels, and to determine whether resistin polymorphisms are associated with resistin levels and lipid profile in prepubertal children and adolescents. The single nucleotide polymorphisms (SNPs) rs1862513 and rs10401670 were analyzed in 442 randomly selected 6- to 8-year-old children and 827 children aged 12–16 years. Anthropometric data were recorded. Lipid profile was determined using standard methods. Serum resistin levels were measured using a multiplexed bead immunoassay. Resistin polymorphisms were determined by TaqMan® allelic discrimination assays. A relationship was found between serum levels of resistin and the SNP rs10401670 in 6- to 8-year-old boys. SNP rs10401670 was also related to TC and LDL-cholesterol in 12- to 16-year-old boys and to HDL-C in 12- to 16-year-old girls. SNP rs1862513 was not related to any of the studied variables. Serum resistin levels were significantly and negatively associated with ApoAI levels in 12- to 16-year-old girls. A SNP in the 3′UTR region of RETN (rs10401670) is associated with resistin levels and lipid profile in children, showing different associations depending on age and gender.

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Acknowledgments

The article is dedicated to the late Prof. Manuel de Oya as the warmest homage to his memory. Prof. de Oya designed the Four Province Study and the ideas reflected in our work can be traced back to his. This work was supported by a grant from the Fondo de Investigación Sanitaria (FIS 11/00344) and Biobank grant FEDER RD09/0076/00101. The contract of C Garcés is co-financed by the Fondo de Investigación Sanitaria. Lorena Ortega and Pilar Navarro are fellows of the Conchita Rábago Foundation. We thank Oliver Shaw for his revision of our manuscript.

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Authors and Affiliations

  1. Lipid Research Laboratory, IIS-Fundación Jiménez Díaz, Avda. Reyes Católicos, 2., 28040, Madrid, Spain

    Lorena Ortega, Pilar Navarro, Pía Riestra & Carmen Garcés

  2. Department of Pediatrics, IIS-Fundación Jiménez Díaz, Madrid, Spain

    Teresa Gavela-Pérez & Leandro Soriano-Guillén

Authors
  1. Lorena Ortega
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  2. Pilar Navarro
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  3. Pía Riestra
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  4. Teresa Gavela-Pérez
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  5. Leandro Soriano-Guillén
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  6. Carmen Garcés
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Corresponding author

Correspondence to Carmen Garcés.

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This article is dedicated to the late Prof. Manuel de Oya, as the warmest homage to his memory.

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Ortega, L., Navarro, P., Riestra, P. et al. Association of resistin polymorphisms with resistin levels and lipid profile in children. Mol Biol Rep 41, 7659–7664 (2014). https://doi.org/10.1007/s11033-014-3658-8

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  • DOI: https://doi.org/10.1007/s11033-014-3658-8

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