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Nanofiber-based polyethersulfone scaffold and efficient differentiation of human induced pluripotent stem cells into osteoblastic lineage

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Abstract

Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential for regenerative medicine and tissue engineering applications. In the present study, osteogenic differentiation of human iPSCs was evaluated on polyethersulfone (PES) nanofibrous scaffold. According to the results, higher significant expressions of common osteogenic-related genes such as runx2, collagen type I, osteocalcin and osteonectin was observed in PES seeded human iPSCs compared with control. Alizarin red staining and alkaline phosphatase activity of differentiated iPSCs demonstrated significant osteoblastic differentiation potential of these cells. In this study biocompatibility of PES nanofibrous scaffold confirmed by flattened and spreading morphology of iPSCs under osteoblastic differentiation inductive culture. Taking together, nanofiber-based PES scaffold seeded iPSCs showed the highest capacity for differentiation into osteoblasts-like cells. These cells and PES scaffold were demonstrated to have great efficiency for treatment of bone damages and lesions.

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Acknowledgments

This study was supported by Stem Cell Technology Research Center, Tehran, Iran.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Abdolreza Ardeshirylajimi, Kazem Parivar & Parichehr Yaghmaie

  2. Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

    Saman Hosseinkhani

  3. Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran

    Masoud Soleimani

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  1. Abdolreza Ardeshirylajimi
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  2. Saman Hosseinkhani
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  3. Kazem Parivar
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  4. Parichehr Yaghmaie
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  5. Masoud Soleimani
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Correspondence to Masoud Soleimani.

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Ardeshirylajimi, A., Hosseinkhani, S., Parivar, K. et al. Nanofiber-based polyethersulfone scaffold and efficient differentiation of human induced pluripotent stem cells into osteoblastic lineage. Mol Biol Rep 40, 4287–4294 (2013). https://doi.org/10.1007/s11033-013-2515-5

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  • DOI: https://doi.org/10.1007/s11033-013-2515-5

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