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Cultivar-specific kinetics of chitinase induction in soybean roots during exposure to arsenic

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Abstract

The kinetics of defense responses was studied in soybean exposed to ecologically relevant concentrations of arsenic for 96 h. In the roots of two soybean cultivars with contrasting tolerance to this metalloid there were observed differences in basal levels of membrane lipid peroxidation as well as a significantly different course of peroxidation upon exposure to As. The different course of stress was reflected in the accumulation of defense components. The responses of individual chitinase isoforms were studied since these enzymes had previously been shown to be stable components of defense against metals. The kinetics and magnitude of accumulation of the three isoforms during exposure to As significantly differed within as well as between the studied cultivars. Furthermore, accumulation of these isoforms appeared to be related to oxidative status in the root tissue. The timing of induced responses is likely to be important for efficient defense against metal(oid) pollution in environment.

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Acknowledgments

This work was supported by grant from the Slovak Grant Agency VEGA No. 2/0062/11 and UGA/VII/27/2012. The authors are grateful to the anonymous reviewers for their comments that helped to improve the earlier versions of this manuscript.

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Authors and Affiliations

  1. Department of Botany and Genetics, Faculty of Natural Sciences, The Constantine Philosopher University, Nábrežie mládeže 91, 949 74, Nitra, Slovak Republic

    Patrik Mészáros, Roman Kuna & Beáta Piršelová

  2. Department of Mathematics, Faculty of Natural Sciences, Constantine the Philosopher University, Tr. A. Hlinku 1, 949 74, Nitra, Slovak Republic

    Ľubomír Rybanský & Anna Tirpáková

  3. Plant Production Research Centre Piešťany, Bratislavská cesta 122, 921 68, Piešťany, Slovak Republic

    Pavol Hauptvogel

  4. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07, Nitra, Slovak Republic

    Jana Libantová, Jana Moravčíková & Ildikó Matušíková

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  1. Patrik Mészáros
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  2. Ľubomír Rybanský
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Correspondence to Ildikó Matušíková.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Online Resource 1 Effect of arsenic on the fresh- and dry weight of soybean roots (DOC 292 kb)

Online Resource 2 Effect of arsenic on cell viability of soybean roots (DOC 270 kb)

Online Resource 3 Effect of arsenic on the amount of hydrogen peroxide in soybean roots (DOC 281 kb)

Online Resource 4 Effect of arsenic on the rate of lipid peroxidation in soybean roots (DOC 37 kb)

Online Resource 5 Effect of arsenic on the proline content of soybean roots (DOC 316 kb)

Online Resource 6 Effect of arsenic on the accumulation of chitinases in soybean roots (DOC 1229 kb)

11033_2012_2271_MOESM7_ESM.doc

Online Resource 7 Correlation analyses on interaction of certain parameters of stress- and defense during exposure of soybean roots to arsenic (DOC 83 kb)

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Mészáros, P., Rybanský, Ľ., Hauptvogel, P. et al. Cultivar-specific kinetics of chitinase induction in soybean roots during exposure to arsenic. Mol Biol Rep 40, 2127–2138 (2013). https://doi.org/10.1007/s11033-012-2271-y

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  • DOI: https://doi.org/10.1007/s11033-012-2271-y

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