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Molecular cloning, characterization and expression of cDNA encoding translationally controlled tumor protein (TCTP) from Jatropha curcas L

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Abstract

A cDNA encoding translationally controlled tumor protein (TCTP) of Jatropha curcas L., JcTCTP, was isolated from an endosperm cDNA library. JcTCTP consisted of a 5′ untranslated region (UTR) of 526 bp, a 3′ UTR of 377 bp and an open reading frame (ORF) of 507 bp, encoding a protein of 168 amino acid residues, which contained two signature sequences of TCTP family. Its deduced amino acid sequence was similar to the other known plants TCTPs in a range of 77.4–92.3%. Expression of JcTCTP was the highest in the stem, endosperm at embryo formation stage and embryo of J. curcas tissues, and the lowest in the endosperm at seminal leaf embryo stage and flower, demonstrating a pattern of temporal and spatial specific expression.

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Acknowledgments

This research is supported by “11th Five-Year Plan” to support science and technology project (NO.2006BAD07A04, NO.2007BAD50B05), Ministry of Science and Technology international cooperation project (NO.2006DFB63400), National Natural Science Foundation project on the surface (NO.30670204), and the Youth Science and Technology Fund of Sichuan Province (No. 07ZQ026-039).

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Author notes

  1. Jinping Zhang

    Present address: Shanghai Entry-Exit Inspection and Quarantine Bureau, 200135, Shanghai, China

  2. Luding Jiang

    Present address: Institute of Materia Medica, College of Pharmay, Third Military Medical University, 400038, Chongqing, China

Authors and Affiliations

  1. College of Life Sciences, Sichuan University, 610064, Chengdu, China

    Xiaobo Qin, Fan Gao, Jinping Zhang, Jihai Gao, Sha Lin, Yong Wang, Luding Jiang, Yi Liao, Lina Wang, Yongjiong Jia, Lin Tang, Ying Xu & Fang Chen

  2. Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California, Riverside, CA, 92521, USA

    Xiaobo Qin

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  1. Xiaobo Qin
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Correspondence to Ying Xu or Fang Chen.

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Qin, X., Gao, F., Zhang, J. et al. Molecular cloning, characterization and expression of cDNA encoding translationally controlled tumor protein (TCTP) from Jatropha curcas L. Mol Biol Rep 38, 3107–3112 (2011). https://doi.org/10.1007/s11033-010-9980-x

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  • DOI: https://doi.org/10.1007/s11033-010-9980-x

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