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Expression of cytokeratin 19 and matrix metalloproteinase 2 predicts lymph node metastasis in hepatocellular carcinoma

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Abstract

The purpose of this study was to assess the value of cytokeratin 19 (CK19) and matrix metalloproteinase 2 (MMP-2) in predicting lymph node metastasis (LNM) and survival after curative resection in hepatocellular carcinoma (HCC) patients. Expression of CK19 and MMP-2 in tumor tissue was assessed through immunohistochemical staining of tissue microarrays (TMAs), which were constructed using samples from HCC patients with (n = 123) and without (n = 145) LNM. Positive CK19 expression was correlated with LNM (P < 0.001), satellite lesions (P = 0.016), and lymph node location (P = 0.039). High MMP-2 expression correlated with LNM (P < 0.001), UICC T stage (P = 0.023), and Edmondson grade (P = 0.022). Moreover, CK19 expression correlated with MMP-2 expression (P = 0.033). CK19 and MMP-2 expression were predictive of HCC LNM (AUC: 0.640; 95% CI: 0.572–0.707; P < 0.001 and AUC: 0.611; 95% CI: 0.544–0.679; P = 0.002, respectively). CK19 and MMP-2 expression were independent prognostic factors for disease-free survival (P = 0.031 and P = 0.012, respectively) and overall survival (P = 0.013 and P = 0.018, respectively) in HCC patients with LNM. CK19 expression (P < 0.001), MMP-2 expression (P = 0.006), and UICC T stage (P < 0.001) were independent risk factors for developing LNM in HCC. These findings show that CK19 and MMP-2 expression may be beneficial in predicting HCC LNM and survival.

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Authors and Affiliations

  1. Department of Radiation Oncology, Zhongshan Hospital, Fudan University, 136 Yi Xue Yuan Road, Shanghai, 200032, China

    Zuo-Lin Xiang & Zhao-Chong Zeng

  2. Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, 200032, China

    Zhao-You Tang, Jia Fan & Hui-Chuan Sun

  3. Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China

    Yun-Shan Tan

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  1. Zuo-Lin Xiang
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  2. Zhao-Chong Zeng
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  4. Jia Fan
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Correspondence to Zhao-Chong Zeng.

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Xiang, ZL., Zeng, ZC., Tang, ZY. et al. Expression of cytokeratin 19 and matrix metalloproteinase 2 predicts lymph node metastasis in hepatocellular carcinoma. Mol Biol Rep 38, 3531–3539 (2011). https://doi.org/10.1007/s11033-010-0463-x

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  • DOI: https://doi.org/10.1007/s11033-010-0463-x

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