Abstract
Recent studies have shown that, in mammals, the highly expressed genes have shorter gene length and their protein products have relatively lower evolutionary rates. However, the global relationship between genes’ expression level and their features such as tissue-specificity, function and protein subcellular localization has not been investigated extensively, especially in mammalian. In order to solve it, we analysed 8,570 genes across 46 human tissues. Our results suggest that widely expressed genes have higher mean expression levels than tissue-specific ones and genes encoding zinc-finger proteins have low expression levels similar to that of DNA-binding proteins. In the analysis of protein subcellular localization, it is shown that nuclear and Golgi apparatus proteins have lower mean expression levels than those of mitochondria, endoplasmic reticulum and membrane proteins, while genes encoding cytoplasm and extracellular components display the highest expression levels. When comparing the gene expression levels and the number of expressed genes in different tissues, we found that some tissues have less active genes while single gene encodes relatively more transcripts. Taken together, gene expression levels are clearly correlated with their tissue-specificity, function and protein subcellular localization, and are highly conserved during evolution.
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Acknowledgments
This work was supported by the National 973 program of China (2004CB518605), the National 863 project of China (2006AA020501), the National Key Sci-Tech Special Project of China (2008ZX10002-020), the Project of the Shanghai Municipal Science and Technology Commission (03dz14086) and the National Natural Science foundation of China (30024001, 30771188).
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The following additional data include three tables. (1) Key statistics of the box-plot representation of the distribution of expression level of ranked genes which have been visualized in Fig. 1a. (2) Detailed information of highly expressed tissue-specific genes (ranks 1–4); (3) Gene number and total AD in tissues which have been illustrated in Fig. 3a.
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Li, Q., Liu, X., He, Q. et al. Systematic analysis of gene expression level with tissue-specificity, function and protein subcellular localization in human transcriptome. Mol Biol Rep 38, 2597–2602 (2011). https://doi.org/10.1007/s11033-010-0400-z
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DOI: https://doi.org/10.1007/s11033-010-0400-z