Abstract
Calpains are a ubiquitous cytoplasmic cysteine protease, the activity of which is absolutely dependent on calcium. This proteolytic system degrades myofibrillar protein under post-mortem conditions and appears to be the primary enzyme in the postmortem tenderization process. In the present study a new single nucleotide polymorphism was found in the bovine CAPNS1 gene exon 11 coding for the 3′UTR. Transition C → T at position 6536 was detected and identified using PCR-SSCP and DNA sequencing techniques, and then analysed with PCR-RFLP using MboII nuclease. The genotype frequencies and alleles distribution were studied in 190 bulls including, Charolaise, Hereford, Limousine, Simmental, Polish Red and Fresian breeds.
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Acknowledgments
This study was funded by the Ministry of Scientific Research and Information Technology grants No. 3 P06D01825, N N311 034834 and the IGHZ project S.V.9.
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Juszczuk-Kubiak, E., Flisikowski, K. & Wicińska, K. A new SNP in the 3′UTR region of the bovine calpain small subunit (CAPNS1) gene. Mol Biol Rep 37, 473–476 (2010). https://doi.org/10.1007/s11033-009-9654-8
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DOI: https://doi.org/10.1007/s11033-009-9654-8