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An improved design of PCR primers for detection of human T cell receptor β chain repertoire

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Abstract

Analysis of T cell receptor β variable region (TCRBV) gene rearrangement is useful for clonal assessment of abnormal T cell proliferations in various diseases. However, most primer panels previously used can only amplify the third complementarity-determining region. Following IMGT database we established a panel of primers, which can amplify entire sequences of all functional TCRBV families. To confirm the usefulness of this panel of primers, we detected different TCRBV repertoires. In 15 healthy donors, most of the BV families were expressed and appeared as a Gaussian distribution. 13 acute myeloid leukemia patients showed monoclonal or oligoclonal changes of BV15 family, and some of them also had monoclonal or oligoclonal expansion of BV2, BV4, BV6 or BV13 families. In one patient after allo-hematopoietic stem cell transplantation, monoclonal proliferation of BV10 family occurred during graft-versus-host disease. In conclusion, this panel of primers improves our abilities to analyze TCRBV repertoire changes in related diseases.

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Acknowledgements

The authors thank all members in the group in Department of Hematology/Oncology, Peking University First Hospital, for their outstanding advice about experimental design. The authors also thank the colleagues in Beijing Daopei Hospital, for patient samples and their suggestions and technical assistance with genescan. This work was supported by China National Natural Science Foundation 30470939 and International Collaboration on Major Topics from China National Ministry of Science and Technology 2006DFB31430.

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Authors and Affiliations

  1. Department of Hematology/Oncology, Center for Medical Genetics, Peking University First Hospital, Peking University, No. 8, Xishiku Street, Beijing, 100034, China

    Yuan Ou, Ying Zhang, Bin Chu & Ping Zhu

  2. Beijing Daopei Hospital, No.12, Yuquan Street, Beijing, 100039, China

    Chunrong Tong, Peng Cai, Jiangying Gu, Yan Liu, Hongxing Liu, He Wang & Ping Zhu

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  1. Yuan Ou
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  2. Chunrong Tong
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  3. Ying Zhang
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  4. Peng Cai
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  5. Jiangying Gu
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  6. Yan Liu
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  7. Hongxing Liu
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  8. He Wang
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  9. Bin Chu
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  10. Ping Zhu
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Correspondence to Ping Zhu.

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Ou, Y., Tong, C., Zhang, Y. et al. An improved design of PCR primers for detection of human T cell receptor β chain repertoire. Mol Biol Rep 36, 145–152 (2009). https://doi.org/10.1007/s11033-008-9328-y

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  • DOI: https://doi.org/10.1007/s11033-008-9328-y

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