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Identification of the cyclin D1b mRNA variant in mouse

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Abstract

Cyclin D1 plays a key regulatory role during the G1 phase of the cell cycle and its gene is amplified and over-expressed in many cancers. The cyclin D1b mRNA variant was established in human cells and recent functional analyses revealed that its protein product harbors unique activities in human cancer cells. By performing reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) experiments, we identified the cyclin D1b mRNA variant in mouse. Similar to its human counterpart, the mouse cyclin D1b transcript consists of exon 1, 2, 3, 4 and part of intron 4, and contains a long open reading frame (ORF). The predicted peptide from this ORF is 34-amino acid longer than the human cyclin D1b. The expression of this mouse mRNA variant was investigated. It appears to be expressed ubiquitously and differentially in various mouse cell lines and tissues and its level might be proportional to that of the canonical endogenous cyclin D1a mRNA.

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Acknowledgement

This work was supported by NIH grant R01CA100864 (J.D.Liao).

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Authors and Affiliations

  1. Karmanos Cancer Institute, Wayne State University, Detroit, MI, 48201, USA

    Jack Wu, Aliccia Bollig & Archana Thakur

  2. Hormel Institute, University of Minnesota, Austin, MN, 55912, USA

    Si-hung Wu & D. Joshua Liao

Authors
  1. Jack Wu
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  2. Si-hung Wu
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  3. Aliccia Bollig
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  4. Archana Thakur
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  5. D. Joshua Liao
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Correspondence to D. Joshua Liao.

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Wu, J., Wu, Sh., Bollig, A. et al. Identification of the cyclin D1b mRNA variant in mouse. Mol Biol Rep 36, 953–957 (2009). https://doi.org/10.1007/s11033-008-9267-7

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  • DOI: https://doi.org/10.1007/s11033-008-9267-7

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