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Organization of the interferon-inducible 2′,5′-oligoadenylate-dependent ribonuclease L (RNase L) gene of mouse

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Abstract

Interferons (IFNs) induce a 2′,5′-oligoadenylate (2-5A)-dependent ribonuclease L (RNase L) following virus-infection of mammalian cells. RNase L degrades both viral and cellular RNAs and restricts virus-proliferation. We have studied organization of RNase L gene in genomic DNA from the mouse liver by Southern blot analysis. Several BamHI, BglII, EcoRI, HincII, HindIII, NcoI, PstI, SacI, and XbaI restriction fragments hybridized to 32P-labeled mouse RNase L cDNA and the 5′-proximal exon probes. Mouse RNase L gene exists as a single copy (>16 kb DNA) gene. A 5 kb HindIII and a 2.5 kb EcoRI DNA were detected as 5′-upstream DNA of the gene which may contain mouse RNase L promoter. Our results will help studying mouse RNase L gene promoter in further details.

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Abbreviations

IFN:

Interferon

2-5A:

2′,5′-oligoadenylate

cDNA:

DNA complementary to RNA

kb:

Kilobase(s) or 1000 bp

ISGs:

IFN-stimulated gene(s)

dsRNA:

Double-stranded RNA

JAK:

Janus kinase

STAT:

Signal transducer and activator of transcription

PKR:

dsRNA-dependent protein kinase

OAS:

2′,5′-oligoadenylate synthetase

bp:

Base pair

SDS:

Sodium dodecyl sulphate

ng:

Nanogram

cpm:

Counts per minute

μg:

Microgram

pg:

Picogram

UV:

Ultraviolet

kDa:

Kilo Dalton

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Acknowledgements

The kind gift of mouse RNase L cDNA (pZB1) plasmid from Prof. R. H. Silverman, Cleveland Clinic Foundation, OH, U.S.A and financial support from the Department of Science and Technology (D.S.T.) and “University of potential for excellence (UPOE)” program of the University Grants Commission (U.G.C.), Govt. of India to P.C.R. through J.N.U. as well as a Junior and Senior Research Fellowship to M.P. from U.G.C. are gratefully acknowledged.

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  1. Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

    Mitali Pandey & Pramod C. Rath

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  1. Mitali Pandey
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  2. Pramod C. Rath
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Correspondence to Pramod C. Rath.

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Pandey, M., Rath, P.C. Organization of the interferon-inducible 2′,5′-oligoadenylate-dependent ribonuclease L (RNase L) gene of mouse. Mol Biol Rep 34, 97–104 (2007). https://doi.org/10.1007/s11033-006-9020-z

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  • DOI: https://doi.org/10.1007/s11033-006-9020-z

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