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Localization of OsTLP27 in thylakoid lumen is required for accumulation of photosynthetic proteins in rice

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Abstract

We previously identified an AtTLP homolog, OsTLP27, in rice, whose overexpression improves chloroplast ultrastructure and photochemical efficiency. However, its molecular role in photosystem (PS) function and the related signaling pathway remain to be elucidated. In this study, using RNAi strategy, OsTLP27-RNAi transgenic plants displayed normal phenotype in medium containing sucrose, but showed growth retardation when transferred to soil under natural growth conditions. Gene expression showed that chloroplast-related genes were affected, and Western blot analysis revealed that accumulation of photosynthetic proteins was reduced significantly, indicating that OsTLP27 was important to normal PS function. Chloroplast subfraction and trypsin protection analysis showed that OsTLP27 localized in the thylakoid lumen. A yeast two-hybrid experiment revealed that OsTLP27 interacts with ribosomal protein L31 and an aldo–keto reductase (AKR) family protein. Our results suggested that OsTLP27 functions in photosynthetic protein accumulation by interacting with L31 and AKR.

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Acknowledgments

This work was supported by the National Genetically Modified Organisms Breeding Major Projects (2009ZX08009-109B), the National Natural Science Foundation of China (31271685) and the National Key Technology R&D Program of China (2011BAD35B02-05) as well as the Public Experiment Center of State Bioindustrial Base (Chongqing).

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    1. Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400030, China

      ZhenHui Kang, JunLi Huang, HanYan Zou, GuangChao Zang & GuiXue Wang

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    1. ZhenHui Kang
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    2. JunLi Huang
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    3. HanYan Zou
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    Correspondence to GuiXue Wang.

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    ZhenHui Kang and JunLi Huang have contributed equally to this work.

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    Kang, Z., Huang, J., Zou, H. et al. Localization of OsTLP27 in thylakoid lumen is required for accumulation of photosynthetic proteins in rice. Mol Breeding 35, 153 (2015). https://doi.org/10.1007/s11032-015-0338-x

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