Abstract
The use of resistant cultivars is the most effective method for controlling sudden death syndrome (SDS), caused by Fusarium solani f. sp. glycines (FSG) (syn. Fusarium virguliforme Akoi, O’Donnell, Homma and Lattanzi), in soybean [Glycine max (L.) Merr.]. Previous research has led to the identification of soybean genotypes with partial resistance to SDS and quantitative trait loci (QTL) controlling this resistance. The objective of our study was to map QTL conferring SDS resistance in populations developed from the crosses Ripley × Spencer (R×S-1) and PI 567374 × Omaha (P×O-1). Both Ripley and PI 567374 have partial resistance to SDS and Spencer and Omaha are susceptible. The R×S-1 population was evaluated for SDS resistance in three field environments and the P×O-1 population was greenhouse evaluated. Three SDS resistance QTL were mapped in the R×S-1 population and two in the P×O-1 population. One resistance QTL was mapped to the same location on linkage group (LG) D2 in both backgrounds. This QTL was then tested in a population of F2 plants developed through one backcross (BC1F2) in the PI 567374 source and in a population of F8 plants derived from a heterozygous F5 plant in the Ripley source. The LG D2 QTL was also significant in confirmation populations in both resistant backgrounds. Since none of the SDS resistance QTL identified in the R×S-1 or P×O-1 populations mapped to previously reported SDS resistance regions, these new QTL should be useful sources of SDS resistance for soybean breeders.
Abbreviations
- BC:
-
Backcross
- DI:
-
Disease incidence
- DS:
-
Disease severity
- DX:
-
Disease index
- LG:
-
Linkage group
- P×O:
-
PI 567374 × Omaha cross
- QTL:
-
Quantitative trait locus (loci)
- R×S:
-
Ripley × Spencer cross
- SDS:
-
Sudden death syndrome
References
Arahana VS, Graef GL, Specht JE, Steadman JR, Eskridge KM (2001) Identification of QTLs for resistance to Sclerotinia sclerotiorum in soybean. Crop Sci 41:180–188
Chang SJC, Doubler TW, Kilo V, Suttner R, Klein J, Schmidt ME, Gibson PT, Lightfoot DA (1996) Two additional loci underlying durable field resistance to soybean sudden death syndrome (SDS). Crop Sci 36:1684–1688
Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971
Cooper RL, Martin RJ, McBlain BA, Fioritto RJ, St. Martin SK, Calip-DuBois A, Schmitthenner AF (1990) Registration of Ripley soybean. Crop Sci 30:963
Cregan PB, Quigley CV (1997) Simple sequence repeat DNA marker analysis. In: Caetano-Anolles G, Gresshoff PM (eds) DNA markers: Protocols, applications and overviews, J. Wiley and Sons, New York, pp 173–185
Farias Neto AF, Hartman GL, Pedersen WL, Li S, Bollero GA, Diers BW (2006) Irrigation and inoculation treatments that increase the severity of soybean sudden death syndrome in the field. Crop Sci 46:2547–2554
Fehr WR, Caviness CE, Burmood DT, Pennington JS (1971) Stage of development descriptions for soybeans, Glycine max (L.) Merrill. Crop Sci 11:929–931
Fronza V, Vello NA, Camargo LEA (2002) Presenca de QTL’s responsaveis pela resistencia a Fusarium solani f. sp. glycines em sete cultivares de soja brasileiras. In: Congresso Brasileiro de soja, 2, abstracts. Foz do Iguacu, PR. p. 29
Germplasm Resources Information Network (2006) USDA-ARS, Beltsville, MD. http://www.ars-grin.gov. Cited 20 Aug 2006
Hallauer AR, Miranda Filho JB (1988) Quantitative genetics in maize breeding. Iowa State University Press, Ames, IA
Hartman GL, Gardner ME, Hymowitz T, Naidoo GC (2000) Evaluation of perennial glycine species for resistance to soybean fungal pathogens that cause sclerotinia stem rot and sudden death syndrome. Crop Sci 40:545–549
Hartman GL, Huang YH, Nelson RL, Noel GR (1997) Germplasm evaluation of Glycine max for resistance to Fusarium solani, the causal organism of sudden death syndrome. Plant Dis 81:515–518
Hartman GL, Noel GR, Gray LE (1994) Occurrence of soybean sudden death syndrome in east-central Illinois and associated yield losses. Plant Dis 79:314–318
Hartwig EE, Young LD, Gibson P (1996) Registration of soybean D83–3349 resistant to sudden death syndrome, soybean cyst nematode, and two root-knot nematode. Crop Sci 36:212
Hnetkovsky N, Chang SJC, Doubler TW, Gibson PT, Lightfoot DA (1996) Genetic mapping of loci underlying field resistance to soybean sudden death syndrome (SDS). Crop Sci 36:393–400
Huang YH, Hartman GL (1996) A semi-selective medium for detecting Fusarium solani, the causal organism of soybean sudden death syndrome. Phytopathology 86:S12
Huang YH, Hartman GL (1998) Reaction of selected soybean genotypes to isolates of Fusarium solani f. sp. glycines and their culture filtrates. Plant Dis 82:999–1002
Iqbal MJ, Meksem K, Njiti VN, Kassem MA, Lightfoot DA (2001) Microsatellite markers identify three additional quantitative trait loci for resistance to soybean sudden-death syndrome (SDS) in Essex × Forrest RILs. Theor Appl Genet 102:187–192
Jansen RC, Stam P (1994) High resolution of quantitative traits into multiple loci via interval mapping. Genetics 136:1447–1455
Jin H, Hartman GL, Nickell CD, Widholm JM (1996) Characterization and purification of a phytotoxin produced by Fusarium solani, the causal agent of soybean sudden death syndrome. Phytopathology 86:277–282
Kabelka EA, Carlson SR, Diers BW (2006) Glycine soja PI 468916 SCN resistance loci associated effects on soybean seed yield and other agronomic traits. Crop Sci 46:622–629
Keim P, Shoemaker RC (1988) A rapid protocol for isolating soybean DNA. Soybean Genet Newsl 15:150–152
Lightfoot DA, Gibson PT, Iqbal MM, Meksem K (2005) Registration of the Essex × Forrest recombinant inbred line mapping population. Crop Sci 45:1678–1681
Meksem K, Doubler TW, Chancharoenchai K, Njiti VN, Chang SJC, Rao Arelli AP, Cregan PB, Gray LE, Gibson PT, Lightfoot DA (1999) Clustering among loci underlying soybean resistance to Fusarium solani, SDS and SCN in near-isogenic lines. Theor Appl Genet 99:1131–1142
Mueller DS (2001) Resistance to Fusarium solani f. sp. glycines, the causal organism of sudden death syndrome of soybean. Ph.D. Dissertation. University of Illinois, Urbana-Champaign
Nickell CD, Bachman MS, Thomas DJ, Cary TR (1998) Registration of Omaha soybean. Crop Sci 38:547
Njiti VN, Johnson JE, Torto TA, Gray LE, Lightfoot DA (2001) Inoculum rates influences selection for field resistance to soybean sudden death syndrome in the greenhouse. Crop Sci 41:1–6
Njiti VN, Meksem K, Iqbal MJ, Johnson JE, Kassem MA, Zobrist KF, Kilo VY, Lightfoot DA (2002) Common loci underlie field resistance to soybean sudden death syndrome in Forrest, Pyramid, Essex, and Douglas. Theor Appl Genet 104:294–300
Njiti VN, Shenaut MA, Suttner RJ, Schmidt ME, Gibson PT (1996) Soybean response to sudden death syndrome: inheritance influenced by cyst nematode resistance in Pyramid × Douglas progenies. Crop Sci 36:1165–1170
Njiti VN, Shenaut MA, Suttner RJ, Schmidt ME, Gibson PT (1998) Relationship between soybean sudden death syndrome disease measures and yield components in F6-derived lines. Crop Sci 38:673–678
Prabhu RR, Njiti VN, Bell Johnson B, Johnson JE, Schmidt ME, Klein JH, Lightfoot DA (1999) Selecting soybean cultivars for dual resistance to soybean cyst nematode and sudden death syndrome using two DNA markers. Crop Sci 39:982–987
Roy KW, Lawrence GW, Hodges HH, McLean KS, Killebrew JF (1989) Sudden death syndrome of soybean: Fusarium solani as incitant and relation of Heterodera glycines to disease severity. Phytopathology 79:191–197
Roy KW, Rupe JC, Hershman DE, Abney TS (1997) Sudden death syndrome of soybean. Plant Dis 81:1100–1111
Rupe JC (1989) Frequency and pathogenicity of Fusarium solani recovered from soybeans with sudden death syndrome. Plant Dis 79:1076–1079
Rupe JC, Hartman GL (1999) Sudden death syndrome. In: Hartman GL, Sinclair JB, Rupe JC (eds) Compendium of soybean diseases. APS Press, St. Paul, Minn, pp 37–39
SAS Institute (2000) SAS user’s guide. SAS Inst., Cary, NC
Schmidt ME, Suttner RJ, Klein JH, Gibson PT, Lightfoot DA, Myers O Jr (1999) Registration of LS-G96 soybean germplasm resistant to soybean sudden death syndrome and soybean cyst nematode race 3. Crop Sci 39:598
Schuster I, Abdelnoor RV, Marin SRR, Carvalho VP, Kiihl RAS, Silva JFV, Sediyama CS, Barros EG, Moreira MA (2001) Identification of a new major QTL associated with resistance to soybean cyst nematode (Heterodera glycines). Theor Appl Gent 102:91–96
Song QJ, Marek LF, Shoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE, Cregan PB (2004) A new genetic linkage map for soybean. Theor Appl Genet 109:122–128
SoyBase (2006) SoyBase. Available at http://soybase.agron.iastate.edu/ (Verified 20 June 2006)
Stephens PA, Nickell CD, Moots CK (1992) Relationship between field and greenhouse reactions of soybean to Fusarium solani. Plant Dis 77:163–166
Stephens PA, Nickell CD, Kolb FL (1993) Genetic analysis of resistance to Fusarium solani in soybean cultivars differing in resistance to Fusarium solani. Crop Sci 33:929–930
Terry LI, Chase K, Jarvik T, Orf J, Mansur L, Lark KG (2000) Soybean quantitative trait loci for resitsance to insects. Crop Sci 40:375–382
Van Ooijen JW, Voorips RE (2001) JoinMap 3.0, Software for the calculation of genetic maps. Plant Research International, Wageninigen, The Netherlands
Van Ooijen JW, Voorips RE, Jansen TC, Maliepaard C (2002) MapQTL 4.0, Software for the calculation of QTL positions on genetic maps. Plant Research International, Wageninigen, The Netherlands
Vick CM, Chong SK, Bond JP, Russin JS (2003) Response of soybean sudden death syndrome to subsoil tillage. Plant Dis 87:629–632
Wang D, Shi J, Carlson SR, Cregan PB, Ward RW, Diers BW (2003) A low-cost, high-throughput polyacrylamide gel electrophoresis system for genotyping with microsatellite DNA markers. Crop Sci 43:1828–1832
Wilcox JR, Roach MT, Abney TS (1989) Registration of Spencer soybean. Crop Sci 29:830–831
Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468
Acknowledgements
This research was supported by grants from the Illinois Soybean Association and the United Soybean Board. A.L. Farias Neto was supported by CAPES, Ministry of Education, Brazil and by Embrapa, Ministry of Agriculture, Brazil.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
de Farias Neto, A.L., Hashmi, R., Schmidt, M. et al. Mapping and confirmation of a new sudden death syndrome resistance QTL on linkage group D2 from the soybean genotypes PI 567374 and ‘Ripley’. Mol Breeding 20, 53–62 (2007). https://doi.org/10.1007/s11032-006-9072-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11032-006-9072-8