Abstract
The astaxanthin synthesis in the yeast Phaffia rhodozyma was shown to depend on the rate of growth occurring in the first two days of cultivation. The growth rate of the yeast culture studied was preset by the cultivation conditions, among which the C : N ratio was decisive. The intense anabolic processes coupled with active culture growth during the first 24 h significantly inhibited the synthesis of the key enzymes involved in astaxanthin synthesis, which led to a marked decrease in the carotenoid production. It was demonstrated that, for the maximum yield of astaxanthin to be obtained from 1 l of nutrient medium, it is necessary to carry out cultivation, beginning with the first day, at a growth rate significantly lower than µmax. The optimum budding rate of the mutant strain Ph. rhodozyma VKPM Y-2409 consistent with the maximum astaxanthin synthesis was determined. The specific astaxanthin productivity of the strain studied was about 7.0 mg/g of dry biomass at a budding rate of <0.5.
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REFERENCES
T.W. Goodwin (1986) ArticleTitleMetabolism, Nutrition, and Function of Carotenoids Annu. Rev. Nutr. 6 273
E.A. Johnson M.J. Lewis C.R. Grau (1980) ArticleTitlePigmentation of Egg Yolks with Astaxanthin from the Yeast Phaffia rhodozyma Poultry Sci. 59 1777–1782
E.A. Johnson T.G. Villia M.J. Lewis (1980) ArticleTitlePhaffia rhodozyma as an Astaxanthin Source in Salmonid Diets Aquaculture 20 123–134
M.T. Kobayashi S. Nagai (1993) ArticleTitleEnhanced Carotenoid Biosynthesis by Oxidative Stress in Acetate-Induced Cyst Cells of a Green Unicellular Alga, Haematococcus pluvialis Appl. Environ. Microbiol. 59 867–873
W.A. Schroeder E.A. Johnson (1995) ArticleTitleSinglet Oxygen and Peroxyl Radicals Regulate Carotenoid Biosynthesis in Phaffia rhodozyma J. Biol. Chem. 270 18374–18379
T. Kakizono M. Kobayashi S. Nagai (1992) ArticleTitleEffect of Carbon/Nitrogen Ratio on Encystment Accompanied with Astaxanthin Formation in a Green Alga, Haematococcus pluvialis J. Ferment. Bioeng. 74 403–405
E.A. Johnson M.J. Lewis (1979) ArticleTitleAstaxanthin Formation by the Yeast Phaffia rhodozyma J. Gen. Microbiol. 115 173–183 Occurrence Handle1:CAS:528:DyaL3cXhsVaqt7s%3D
P.S. Meyer J.C. Preez ParticleDu (1994) ArticleTitleEffect of Culture Conditions on Astaxanthin Production by a Mutant of Phaffia rhodozyma in Batch and Chemostat Culture Appl. Microbiol. Biotechnol. 40 780–785
Prevatt, W.D., Dickson, T.D., and Harris, R.L., Novel Strain of Phaffia rhodozyma Containing High Levels of Astaxanthin, World Patent, 1991, no. 8712855.
P.S. Meyer J.C. Preez ParticleDu (1994) ArticleTitleAstaxanthin Production by a Phaffia rhodozyma Mutant on Grape Juice World J. Microbiol. Biotechnol. 10 178–183
G.-H. An D.B. Schuman E.A. Johnson (1989) ArticleTitleIsolation of Phaffia rhodozyma Mutants with Increased Astaxanthin Content Appl. Environ. Microbiol. 55 116–121
A. Jimenez B. Littlewood J. Davies (1972) Inhibition of Protein Synthesis in Yeast E. Munoz (Eds) Molecular Mechanisms of Antibiotic Action on Protein Biosynthesis and Membranes Elsevier Amsterdam
E.A. Johnson T.G. Villa M.J. Lewis H.J. Phaff (1978) ArticleTitleSimple Method for Isolation of Astaxanthin from the Basidiomycetous Yeast Phaffia rhodozyma Appl. Environ. Microbiol. 35 1155–1159
Vustin, M.M., Belykh, E.N., Kishilova, S.A., and Sineokii, S.P., Effect of the C/N Ratio on the Synthesis of Carotenoids by the Yeast Phaffia rhodozyma, Trudy IX mezhdunar. konf. “Novye informatsionnye tekhnologii v meditsine i ekologii”, Krym, Gurzuf, 1–10 iyunya 2001 g., (Proc. IX Int. Conf. “New Informational Technologies in Medicine and Ecology”, Gurzuf, June 1–10, 2001), pp. 108–109.
S.J. Pirt (1975) Principles of Microbe and Cell Cultivation Blackwell Oxford
R.Y. Stanier E.A. Adelberg J.L. Ingraham (1976) The Microbial World EditionNumber4th ed. Prentice-Hall Englewood Cliffs
Y.A. Rybakov G.I. Bourd (1991) ArticleTitleNitrogen Regulation of Gibberellin Biosynthesis Enzyme Complex in Fusarium moniliforme J. Biotechnol. 21 219–228
Y. Yamane K. Higashida Y. Nakashimada T. Kakizono N. Nishio (1997) ArticleTitleInfluence of Oxygen and Glucose on Primary Metabolism and Astaxanthin Production by Phaffia rhodozyma in Batch and Fed-Batch Cultures: Kinetic and Stoichiometric Analysis Appl. Environ. Microbiol. 63 4471–4478
L.B. Flores-Cotera R. Martin S. Sanchez (2001) ArticleTitleCitrate, a Possible Precursor of Astaxanthin in Phaffia rhodozyma: Influence of Varying Levels of Ammonium, Phosphate and Citrate in a Chemically Defined Medium Appl. Microbiol. Biotechnol. 55 341–347
C. Larsson U.V. Stockar I. Marison L. Gustafsson (1993) ArticleTitleGrowth and Metabolism of Saccharomyces cerevisiae in Chemostat Cultures Under Carbon-, Nitrogen-, or Carbon and Nitrogen-Limiting Conditions J. Bacteriol. 175 4809–4816
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Translated from Mikrobiologiya, Vol. 73, No. 6, 2004, pp. 751–757.
Original Russian Text Copyright © 2004 by Vustin, Belykh, Kishilova.
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Vustin, M.M., Belykh, E.N. & Kishilova, S.A. Relationship between astaxanthin production and the intensity of anabolic processes in the yeast Phaffia rhodozyma. Microbiology 73, 643–649 (2004). https://doi.org/10.1007/s11021-005-0004-0
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DOI: https://doi.org/10.1007/s11021-005-0004-0