Skip to main content
SpringerLink
Log in

Bio-synthesized selenium nanoparticles ameliorate Brain oxidative stress in Parkinson disease rat models

  • Original Article
  • Published:
Metabolic Brain Disease Aims and scope Submit manuscript

Abstract   

Aim

Parkinson disease (PD) is a prevalent central nervous system degenerative condition that impacts elderly people. Recent clinical and experimental study findings have established oxidative stress as one of the main pathogeneses of PD. Selenium, a trace metals with antioxidant effects, might reverse the neurobehavioral impairments and oxidative stress in rats. Thus, the goal of this study was to ascertain if Selenium Nano Particles (SeNPs) are also effective to protect brain cells from oxidative stress or not.

Main methods

SeNPs were synthesized utilizing Ascorbic acid and chitosan as a reducing and stabilizing agent. Next, eight groups (N: 6) of male Wistar rats were randomly assigned and injected by different dosage (0.1, 0,2, and 0.3 mg/kg) of Se and SeNP. Finally, to ascertain the protective benefits of SeNP on PD rats, behavioral evaluation, clinical symptoms, antioxidant activity, and oxidant levels were examined.

Key findings

According to the findings, PD rats' motor functions had developed by SeNP injection. Higher MDA levels and inhibited antioxidant activities (SOD, CAT, and GPX) in lesion group are highlighting the significant role of oxidative stress in dopaminergic neuron death and neurobehavioral abnormalities. SeNP also protect against oxidative stress as compared to the lesion group. The levels of MDA had greatly reduced while the activities of enzymes, TAC, and SeNP both had significantly increased.

Significance

By enhancing antioxidant activity, administration of SeNP can reduce the hazardous consequences of oxidative stress.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Data availability

The data in this article are available to the corresponding author upon reasonable request.

Code availability

Not applicable.

References  

  • Alberio T, Pippione AC, Comi C, Olgiati S, Cecconi D, Zibetti M et al (2012) Dopaminergic therapies modulate the T-CELL proteome of patients with Parkinson’s disease. IUBMB Life 64:846–852

    Article  CAS  PubMed  Google Scholar 

  • Amin KA, Hashem KS, Alshehri FS, Awad ST, Hassan MS (2017) Antioxidant and hepatoprotective efficiency of selenium nanoparticles against acetaminophen-induced hepatic damage. Biol Trace Elem Res 175:136–145

    Article  PubMed  Google Scholar 

  • Amiri H, Hashemy SI, Sabouri Z, Javid H, Darroudi M (2021) Green synthesized selenium nanoparticles for ovarian cancer cell apoptosis. Res Chem Intermed 47:2539–2556

    Article  CAS  Google Scholar 

  • Asl SS, Amiri I, Samzadeh-Kermani A, Abbasalipourkabir R, Gholamigeravand B, Shahidi S (2021) Chitosan-coated Selenium nanoparticles enhance the efficiency of stem cells in the neuroprotection of streptozotocin-induced neurotoxicity in male rats. Int J Biochem Cell Biol 141:106089

    Article  Google Scholar 

  • Balestrino R, Schapira A (2020) Parkinson Disease. Eur Neurol 27:27–42

    Article  CAS  Google Scholar 

  • Behl T, Kaur D, Sehgal A, Singh S, Sharma N, Zengin G et al (2021) Role of monoamine oxidase activity in Alzheimer’s disease: an insight into the therapeutic potential of inhibitors. Molecules 26:3724

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bellucci A, Bubacco L, Longhena F, Parrella E, Faustini G, Porrini V et al (2020) Nuclear Factor-κB dysregulation and α-synuclein pathology: Critical interplay in the pathogenesis of Parkinson’s disease. Front Aging Neurosci 12:68

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Benko I, Nagy G, Tanczos B, Ungvari E, Sztrik A, Eszenyi P et al (2012) Subacute toxicity of nano-selenium compared to other selenium species in mice. Environ Toxicol Chem 31:2812–2820

    Article  CAS  PubMed  Google Scholar 

  • Bigham M, Mohammadipour A, Hosseini M, Malvandi AM, Ebrahimzadeh-Bideskan A (2021) Neuroprotective effects of garlic extract on dopaminergic neurons of substantia nigra in a rat model of Parkinson’s disease: motor and non-motor outcomes. Metab Brain Dis 36:927–937

    Article  CAS  PubMed  Google Scholar 

  • Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O (2012) Oxidative stress and antioxidant defense. World Allergy Organ J 5:9–19

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bisht N, Phalswal P, Khanna PK (2022) Selenium nanoparticles: a review on synthesis and biomedical applications. Materials Advances 3:1415–1431

    Article  CAS  Google Scholar 

  • Chand K, Jiao C, Lakhan MN, Shah AH, Kumar V, Fouad DE et al (2021) Green synthesis, characterization and photocatalytic activity of silver nanoparticles synthesized with Nigella Sativa seed extract. Chem Phys Lett 763:138218

    Article  CAS  Google Scholar 

  • Cheung RCF, Ng TB, Wong JH, Chan WY (2015) Chitosan: an update on potential biomedical and pharmaceutical applications. Mar Drugs 13:5156–5186

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Damier P, Hirsch E, Zhang P, Agid Y, Javoy-Agid F (1993) Glutathione peroxidase, glial cells and Parkinson’s disease. Neuroscience 52:1–6

    Article  CAS  PubMed  Google Scholar 

  • Dawood MA, Basuini MFE, Yilmaz S, Abdel-Latif HM, Kari ZA, Abdul Razab MKA et al (2021) Selenium nanoparticles as a natural antioxidant and metabolic regulator in aquaculture: A review. Antioxidants 10:1364

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • de Farias CC, Maes M, Bonifácio KL, Bortolasci CC, de Souza NA, Brinholi FF et al (2016) Highly specific changes in antioxidant levels and lipid peroxidation in Parkinson’s disease and its progression: disease and staging biomarkers and new drug targets. Neurosci Lett 617:66–71

    Article  PubMed  Google Scholar 

  • Diko CS, Zhang H, Lian S, Fan S, Li Z, Qu Y (2020) Optimal synthesis conditions and characterization of selenium nanoparticles in Trichoderma sp WL-Go culture broth. Mater Chem Phys 246:122583

    Article  CAS  Google Scholar 

  • Draper H, Dhanakoti S, Hadley M, Piche L (2019) Malondialdehyde in biological systems. CRC Press, Cellular antioxidant defense mechanisms, pp 97–109

    Google Scholar 

  • Forman HJ, Zhang H (2021) Targeting oxidative stress in disease: Promise and limitations of antioxidant therapy. Nat Rev Drug Discovery 20:689–709

    Article  CAS  PubMed  Google Scholar 

  • García DS, de Deus FT, Castro ES, Borrué C, Mata M, Vila BS et al (2019) Non-motor symptoms burden, mood, and gait problems are the most significant factors contributing to a poor quality of life in non-demented Parkinson’s disease patients: Results from the COPPADIS Study Cohort. Parkinsonism Relat Disord 66:151–157

    Article  Google Scholar 

  • Gupta RK, Patel AK, Shah N, Choudhary AK, Jha UK, Yadav UC et al (2014) Oxidative stress and antioxidants in disease and cancer: a review. Asian Pac J Cancer Prev 15:4405–4409

    Article  PubMed  Google Scholar 

  • Hussain I, Singh N, Singh A, Singh H, Singh S (2016) Green synthesis of nanoparticles and its potential application. Biotech Lett 38:545–560

    Article  CAS  Google Scholar 

  • Jeevitha D, Amarnath K (2013) Chitosan/PLA nanoparticles as a novel carrier for the delivery of anthraquinone: Synthesis, characterization and in vitro cytotoxicity evaluation. Colloids Surf, B 101:126–134

    Article  CAS  Google Scholar 

  • Jia X, Li N, Chen J (2005) A subchronic toxicity study of elemental Nano-Se in Sprague-Dawley rats. Life Sci 76:1989–2003

    Article  CAS  PubMed  Google Scholar 

  • Kazemi M, Akbari A, Zarrinfar H, Soleimanpour S, Sabouri Z, Khatami M et al (2020) Evaluation of antifungal and photocatalytic activities of gelatin-stabilized selenium oxide nanoparticles. J Inorg Organomet Polym Mater 30:3036–3044

    Article  CAS  Google Scholar 

  • Kazemi M, Akbari A, Sabouri Z, Soleimanpour S, Zarrinfar H, Khatami M et al (2021) Green synthesis of colloidal selenium nanoparticles in starch solutions and investigation of their photocatalytic, antimicrobial, and cytotoxicity effects. Bioprocess Biosyst Eng 44:1215–1225

    Article  CAS  PubMed  Google Scholar 

  • Krukouskaya T, Sinevich E, Zagorsky S, Melnov S, Golovach M (2013) Multielement profiles of children in urban environmental context. Ecol Urban Areas 2013:610

    Google Scholar 

  • Lee HM, Koh S-B (2015) Many faces of Parkinson’s disease: non-motor symptoms of Parkinson’s disease. J Movement Disord 8:92

    Article  Google Scholar 

  • Levy OA, Malagelada C, Greene LA (2009) Cell death pathways in Parkinson’s disease: proximal triggers, distal effectors, and final steps. Apoptosis 14:478–500

    Article  PubMed  PubMed Central  Google Scholar 

  • Li Z, Yao Q, Tian Y, Jiang Y, Xu M, Wang H et al (2022) Trehalose protects against cisplatin-induced cochlear hair cell damage by activating TFEB-mediated autophagy. Biochem Pharmacol 197:114904

    Article  CAS  PubMed  Google Scholar 

  • Li J, Wuliji O, Li W, Jiang Z-G, Ghanbari HA (2013) Oxidative stress and neurodegenerative disorders. Int J Mol Sci 14:24438–75

    Article  PubMed  PubMed Central  Google Scholar 

  • Liang P, Qian D, Zhenfeng G, Yongyi C, Yi S, Liang L (2021) Biological selenium nano-particles modify immune responses of macrophages exposed to bladder tumor antigens. J Cluster Sci 32:1623–1633

    Article  CAS  Google Scholar 

  • Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D et al (2018) Oxidative stress, aging, and diseases. Clin Interv Aging 13:757

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Martínez Y, Li X, Liu G, Bin P, Yan W, Más D et al (2017) The role of methionine on metabolism, oxidative stress, and diseases. Amino Acids 49:2091–2098

    Article  PubMed  Google Scholar 

  • Mohan CO, Gunasekaran S, Ravishankar C (2019) Chitosan-capped gold nanoparticles for indicating temperature abuse in frozen stored products. npj Science of Food 3:2

    Article  PubMed  PubMed Central  Google Scholar 

  • Nguyen NT, Nguyen NT, Nguyen VA (2020) In situ synthesis and characterization of ZnO/chitosan nanocomposite as an adsorbent for removal of Congo red from aqueous solution. Adv Polym Technol 2020:1–8

    Google Scholar 

  • Percário S, da Silva Barbosa A, Varela ELP, Gomes ARQ, Ferreira MES, de Nazaré Araújo Moreira T et al (2020) Oxidative stress in parkinson’s disease: Potential benefits of antioxidant supplementation. Oxidative Med Cell Longev 2020:2360872

  • Phan TTV, Phan DT, Cao XT, Huynh T-C, Oh J (2021) Roles of chitosan in green synthesis of metal nanoparticles for biomedical applications. Nanomaterials 11:273

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ramamurthy C, Sampath KS, Arunkumar P, Kumar MS, Sujatha V, Premkumar K et al (2013) Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess Biosyst Eng 36:1131–1139

    Article  CAS  PubMed  Google Scholar 

  • Salaramoli S, Joshaghani H, Hashemy SI (2022) Selenium effects on oxidative stress-induced calcium signaling pathways in Parkinson’s disease. Indian J Clin Biochem 37:257–266

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shehabeldine A, Hasanin M (2019) Green synthesis of hydrolyzed starch–chitosan nano-composite as drug delivery system to gram negative bacteria. Environ Nanotechnol Monitoring Manag 12:100252

    Article  Google Scholar 

  • Sobolev O, Gutyj B, Petryshak R, Pivtorak J, Kovalskyi Y, Naumyuk A et al (2018) Biological role of selenium in the organism of animals and humans. Ukrainian J Ecol 8:654–665

    Article  Google Scholar 

  • Thirumavalavan M, Huang K-L, Lee J-F (2013) Preparation and morphology studies of nano zinc oxide obtained using native and modified chitosans. Materials 6:4198–4212

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Trist BG, Hare DJ, Double KL (2019) Oxidative stress in the aging substantia nigra and the etiology of Parkinson’s disease. Aging Cell 18:e13031

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Trojanowski JQ, LEE VMY (2003) Parkinson’s disease and related α-synucleinopathies are brain amyloidoses. Ann New York Acad Sci 991:107–10

    Article  CAS  Google Scholar 

  • Usman MS, Ibrahim NA, Shameli K, Zainuddin N, Yunus WMZW (2012) Copper nanoparticles mediated by chitosan: synthesis and characterization via chemical methods. Molecules 17:14928–14936

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Vyas J, Rana S (2017) Antioxidant activity and green synthesis of selenium nanoparticles using allium sativum extract. Int J Phytomedicine 9:634–641

    Article  CAS  Google Scholar 

  • Younesian O, Khodabakhshi B, Abdolahi N, Norouzi A, Behnampour N, Hosseinzadeh S et al (2022) Decreased Serum Selenium Levels of COVID-19 Patients in Comparison with Healthy Individuals. Biol Trace Elem Res 200:1562–1567

    Article  CAS  PubMed  Google Scholar 

  • Yue D, Zeng C, Okyere SK, Chen Z, Hu Y (2021) Glycine nano-selenium prevents brain oxidative stress and neurobehavioral abnormalities caused by MPTP in rats. J Trace Elem Med Biol 64:126680

    Article  CAS  PubMed  Google Scholar 

  • Zafar KS, Siddiqui A, Sayeed I, Ahmad M, Salim S, Islam F (2003) Dose-dependent protective effect of selenium in rat model of Parkinson’s disease: neurobehavioral and neurochemical evidences. J Neurochem 84:438–446

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements 

Here, it is acknowledged that the Research Deputy of Mashhad University of Medical Sciences provided financial assistance for this project and Kavosh laboratory for their scientifically supports.

Funding

The research leading to these results received funding from [Research Deputy of Mashhad University of Medical Sciences] under Grant Agreement No [980950].

Author information

Authors and Affiliations

  1. Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Sanaz Salaramoli & Hamed Amiri

  2. Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Sanaz Salaramoli, Hamed Amiri & Seyed Isaac Hashemy

  3. Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran

    Hamid Reza Joshaghani

  4. Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Mahmoud Hosseini

Authors
  1. Sanaz Salaramoli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hamed Amiri
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hamid Reza Joshaghani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mahmoud Hosseini
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seyed Isaac Hashemy
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [Sanaz Salaramoli], [Hamed Amiri] and [Hamidreza Joshaghani]. The first draft of the manuscript was written by [Sanaz Salaramoli] and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Seyed Isaac Hashemy.

Ethics declarations

Ethics approval

Ethical approval was acquired from the Mashhad University of Medical Science Ethics Committee (ethic No: IR.MUMS.MEDICAL.REC.1400.039).

Consent to participate

Not applicable.

Consent for publication

Not applicable.

Conflict of interests

There is no conflict of interest to declare.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Highlights

• 6-OHDA injection impairs behavioral function of the subjects.

• Selenium Nano particle (SeNP) have neuroprotective effects on the rats with Parkinson Disease.

• Neurobehavioral impairments and oxidative stress are protected by green synthesized Selenium Nano particle (SeNP) in a dose-dependent manner.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Salaramoli, S., Amiri, H., Joshaghani, H.R. et al. Bio-synthesized selenium nanoparticles ameliorate Brain oxidative stress in Parkinson disease rat models. Metab Brain Dis 38, 2055–2064 (2023). https://doi.org/10.1007/s11011-023-01222-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11011-023-01222-6

Keywords

Search

Navigation