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The ameliorative role of Physalis pubescens L. against neurological impairment associated with streptozotocin induced diabetes in rats

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Abstract

Neuropathy is considered a critical complication of diabetes mellitus (DM). Scientific studies are needed to relieve these painful complications. The current study aims to estimate the ameliorative role of Physalis juice (PJ) against neurological impairment in streptozotocin (STZ)-induced diabetic rats. Type 1 DM was induced after one week of injecting rats with 55 mg STZ/kg body weight. PJ-treated rats were orally administered 5 ml PJ/kg body weight per day for 28 days after induction of diabetes. A small piece of the cerebral cortex of rats was fixed and used for histopathological investigations. The remaining portion of the cerebral cortex was homogenized for biochemical and molecular analyses. As compared to the controls, STZ-injected rats showed significant elevations in the levels of blood glucose, tumor necrosis factor alfa, interleukin-1β, malondialdehyde, nitric oxide, and expression levels of caspase-3 and B-cell lymphoma-2 associated X-protein. Additionally, remarkable declines in the levels of brain-derived neurotrophic factor, monoamines, B-cell lymphoma-2, glutathione, as well as the activities and gene expression levels of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase in STZ-treated rats were reported. Moreover, some histopathological alterations were observed in the brain cortex of the STZ-treated rats. On the other hand, the administration of PJ substantially reduced the blood glucose and alleviated the above-mentioned alterations in all the studied parameters of the cerebral cortex. In conclusion, an oral administration of 5 ml PJ/kg revealed a neuroprotective action against neurodegenerative diabetes-induced complications in rats, which might be due to the reported antioxidative and anti-inflammatory actions of PJ. Thus, further therapeutic studies are recommended to apply PJ in the treatment regimen of diabetes.

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References

  • Abdel-Ghaffar O, Ali AA, Soliman SA (2018) Protective effect of naringenin against isoniazid-induced adverse reactions in rats. Int J Pharmacol 14(5):667–680. https://doi.org/10.3923/ijp.2018.667.680

    Article  CAS  Google Scholar 

  • Albasher G, Alsaleh AS, Alkubaisi N, Alfarraj S, Alkahtani S, Farhood M, Alotibi N, Almeer R (2020) Red beetroot extract abrogates chlorpyrifos-induced cortical damage in rats. Oxid Med Cell Longev 2020:2963020. https://doi.org/10.1155/2020/2963020

  • Ali AA, Mansour AB, Attia SA (2021) The potential protective role of apigenin against oxidative damage induced by nickel oxide nanoparticles in liver and kidney of male Wistar rat, Rattus norvegicus. Environ Sci Pollut Res. https://doi.org/10.1078/0944711041495254

    Article  Google Scholar 

  • Al-Quraishy S, Dkhil MA, Abdel Moneim AE (2015) Anti-hyperglycemic activity of selenium nanoparticles in streptozotocin-induced diabetic rats. Int J Nanomed 10:6741–6756

    CAS  Google Scholar 

  • Baty RS, Hassan KE, Alsharif KF, El-Hennamy RE, Elmahallawy EK, Hafez MM, Moneim AA, Kassab RB (2020) Neuroprotective role of luteolin against lead acetate-induced cortical damage in rats. Hum Exp Toxicol 39(9):1200–1212. https://doi.org/10.1177/0960327120913094

    Article  CAS  PubMed  Google Scholar 

  • Bouafir Y, Ait-Lounis A, Laraba-Djebari F (2017) Improvement of function and survival of pancreatic beta-cells in streptozotocin-induced diabetic model by the scorpion venom fraction F1. Toxin Rev 36(2):101–108

    Article  CAS  Google Scholar 

  • Çakir Ö, Pekmez M, Çepni E, Candar B, Fidan K (2014) Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells. Food Sci Technol 34(2):422–430

    Article  Google Scholar 

  • Cho NH, Shaw JE, Karuranga S, Huang Y, Da RFJ, Ohlrogge AW, Malanda B (2018) IDF Diabetes Atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045. Diabetes Res Clin Pract 18:271–281

    Article  Google Scholar 

  • Dalise S, Cavalli L, Ghuman H, Wahlberg B, Gerwig M, Chisari C, Ambrosio F, Modo M (2017) Biological effects of dosing aerobic exercise and neuromuscular electrical stimulation in rats. Sci Rep 7(1):1–13

    Article  CAS  Google Scholar 

  • Dewi L, Sulchan M (2018) Potency of cape gooseberry (Physalis Peruviana) juice in improving antioxidant and adiponectin level of high fat diet streptozotocin rat model. Rom J Diabetes Nutr Metab Dis 25(3):253–260. https://doi.org/10.2478/rjdnmd-2018-0029

    Article  CAS  Google Scholar 

  • Dinçel GU, Yildirim S, Oğuz KU (2018) Role of nitric oxide and oxidative stress in pathophysiology of liver injury in streptozotocin-induced type 1 diabetic rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi 65(1):39–50

    Article  Google Scholar 

  • Dong B, An L, Yang X, Zhang X, Zhang J, Tuerhong M, Jin DQ, Ohizumi Y, Lee D, Xu J, Guo Y (2019) Withanolides from Physalis peruviana showing nitric oxide inhibitory effects and affinities with iNOS. Bioorg Chem 87:585–593

    Article  CAS  Google Scholar 

  • El-Beltagi HS, Mohamed HI, Safwat G, Gamal M, Megahed BM (2019) Chemical composition and biological activity of Physalis peruviana L. Gesunde Pflanz 71(2):113–122

    Article  CAS  Google Scholar 

  • Giorgetti M, Negri G (2011) Plants from Solanaceae family with possible anxiolytic effect reported on 19th century’s Brazilian medical journal. Rev Bras Farmacogn 21:772–780

    Article  Google Scholar 

  • Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR (1982) Analysis of nitrate, nitrite, and [15 N] nitrate in biological fluids. Anal biochem 126(1):131–138

    Article  CAS  Google Scholar 

  • He X, Sun J, Huang X (2018) Expression of caspase-3, Bax and Bcl-2 in hippocampus of rats with diabetes and subarachnoid hemorrhage. Exp Therap med 15(1):873–887. https://doi.org/10.3892/etm.2017.5438

    Article  CAS  Google Scholar 

  • Hegazi R, El-Gamal M, Abdel-Hady N, Hamdy O (2015) Epidemiology of and risk factors for type 2 Diabetes in Egypt. Ann Glob Health 81(6):815–816. https://doi.org/10.1016/j.aogh.2015.12.011

    Article  Google Scholar 

  • Huang D, Refaat M, Mohammedi K, Jayyousi A, Al Suwaidi J, Abi Khalil C (2017) Macrovascular complications in patients with diabetes and prediabetes. Biomed Res Int 2017:7839101. https://doi.org/10.1155/2017/7839101

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kang YM, Kim YJ, Park JY, Lee WJ, Jung CH (2016) Mortality and causes of death in a national sample of type 2 diabetic patients in Korea from 2002 to 2013. Cardiovas Diabetol 15(1):131

    Article  Google Scholar 

  • Kim HJ, Jung BH, Yoo KY, Han JW, Um HS, Chang BS, Lee JK (2017) Determination of the critical diabetes duration in a streptozotocin-induced diabetic rat calvarial defect model for experimentation regarding bone regeneration. J Periodontal Implant Sci 47(5):339–350

    Article  CAS  Google Scholar 

  • Martinowich K, Lu B (2008) Interaction between BDNF and serotonin: Role in mood disorders. Neuropsychopharmacology 33:73–83

    Article  CAS  Google Scholar 

  • Mohamed AA, Ali MM, Dorrah MA, Bassal TT (2018) Mediation of inducible nitric oxide and immune-reactive lysozymes biosynthesis by eicosanoid and biogenic amines in flesh flies. Int J Trop Insect Sci 38(1):93–104. https://doi.org/10.1017/S1742758417000315

    Article  Google Scholar 

  • Moneim AE, Bauomy AA, Diab MM, Shata MT, Al-Olayan EM, El-Khadragy MF (2014) The protective effect of Physalis peruviana L. against cadmium-induced neurotoxicity in rats. Biol Trace Elem Res 160(3):392–399

    Article  Google Scholar 

  • Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358

    Article  CAS  Google Scholar 

  • Onaolapo AY, Ayeni OJ, Ogundeji MO, Ajao A, Onaolapo OJ, Owolabi AR (2019) Subchronic ketamine alters behaviour, metabolic indices and brain morphology in adolescent rats: Involvement of oxidative stress, glutamate toxicity and caspase-3-mediated apoptosis. J Chem Neuroanat 96:22–33. https://doi.org/10.1016/j.jchemneu.2018.12.002

    Article  CAS  PubMed  Google Scholar 

  • Pagel P, Blome J, Wolf HU (2000) High-performance liquid chromatographic separation and measurement of various biogenic compounds possibly involved in the pathomechanism of Parkinson’s disease. J Chromatogr B Biomed Sci Appl 746(2):297–304

    Article  CAS  Google Scholar 

  • Prabhakar V, Gupta D, Kanade P, Radhakrishnan M (2015) Diabetes-associated depression: the serotonergic system as a novel multifunctional target. Indian J Pharmacol 47(1):4–10

    Article  CAS  Google Scholar 

  • Rey DP, Ospina LF, Aragón DM (2015) Inhibitory effects of an extract of fruits of Physalis peruviana on some intestinal carbohydrases. Rev Colomb Cienc Quím Farm 44(1):72–89

    Article  CAS  Google Scholar 

  • Robertson SD, Matthies HJ, Owens WA, Sathananthan V, Christianson NS, Kennedy JP, Lindsley CW, Daws LC, Galli A (2010) Insulin reveals Akt signaling as a novel regulator of norepinephrine transporter trafficking and norepinephrine homeostasis. J Neurosci 30(34):11305–11316

    Article  CAS  Google Scholar 

  • Rorsman P, Ashcroft FM (2018) Pancreatic β-cell electrical activity and insulin secretion: of mice and men. Physiol Rev 98(1):117–214

    Article  CAS  Google Scholar 

  • Samarghandian S, Borji A, Farkhondeh T (2017) Attenuation of oxidative stress and inflammation by Portulaca oleracea in streptozotocin-induced diabetic rats. J Evid Based Complement Alternat Med 22(4):562–566

    Article  CAS  Google Scholar 

  • Saperstein S, Chen L, Oakes D, Pryhuber G, Finkelstein J (2009) IL-1beta augments TNF-alpha-mediated inflammatory responses from lung epithelial cells. J Interferon Cytokine Res 29(5):273–284

    Article  CAS  Google Scholar 

  • Sayed AA, Ali AA, Mohamed HR (2018) Fertility enhancing efficacy of Cicer arietinum in male albino mice. Cell Mol Biol 64(4):29–38. https://doi.org/10.14715/cmb/2018.64.4.6

    Article  PubMed  Google Scholar 

  • Simeonova R, Vitcheva V, Kondeva-Burdina M, Popov G, Shkondrov A, Manov V, Krasteva I (2019) Alcesefoliside protects against oxidative brain injury in rats. Rev Bras Farm 29(2):221–227

    Article  CAS  Google Scholar 

  • Sivaraman K, SenthilKumar GP, Sankar P, Bobby Z (2013) Attenuation of oxidative stress, inflammation and insulin resistance by Allium sativum in fructose-fed male rats. J Clin Diagn Res 7:1860–1862. https://doi.org/10.7860/jcdr/2013/6924.3334

    Article  CAS  Google Scholar 

  • Vadivelu R, Vijayvergiya R (2018) Panvascular risk factor – Diabetes. Cor Vasa 60(1):18–29

    Article  Google Scholar 

  • Waisundara VY, Hsu A, Huang D, Tan BKH (2008) Scutellaria baicalensis enhances the anti-diabetic activity of metformin in streptozotocin-induced diabetic Wistar rats. Am J Chin Med 36(3):517–540

    Article  CAS  Google Scholar 

  • Wang A, Wang S, Zhou F, Li P, Wang Y, Gan L, Lin L (2018) Physalin B induces cell cycle arrest and triggers apoptosis in breast cancer cells through modulating p53-dependent apoptotic pathway. Biomed Pharmacother 101:334–341. https://doi.org/10.1016/j.biopha.2018.02.094

    Article  CAS  PubMed  Google Scholar 

  • Wang C, Liu Y, Wang Y, Wei Z, Suo D, Ning G, Wu Q, Feng S, Wan C (2019a) Low–frequency pulsed electromagnetic field promotes functional recovery, reduces inflammation and oxidative stress, and enhances HSP70 expression following spinal cord injury. Mol Med Rep 19(3):1687–1693

    CAS  PubMed  PubMed Central  Google Scholar 

  • Wang J, Cheng C, Xin C, Wang Z (2019b) The antidepressant-like effect of flavonoids from Trigonella foenum-graecum seeds in chronic restraint stress mice via modulation of monoamine regulatory pathways. Molecules 24(6):1105. https://doi.org/10.3390/molecules24061105

    Article  CAS  PubMed Central  Google Scholar 

  • Wang Y, Wang D, Jin Z (2019c) miR–27a suppresses TLR4–induced renal ischemia–reperfusion injury. Mol Med Rep 20(2):967–976

    CAS  PubMed  PubMed Central  Google Scholar 

  • Yang YJ, Yi L, Wang Q, Xie BB, Dong Y, Sha CW (2017) Anti-inflammatory effects of physalin E from Physalis angulata on lipopolysaccharide-stimulated RAW 264.7 cells through inhibition of NF-κB pathway. Immunopharmacol Immunotoxicol 39(2):74–79

    Article  CAS  Google Scholar 

  • Yin C, Huang GF, Sun XC, Guo Z, Zhang JH (2016) Tozasertib attenuates neuronal apoptosis via DLK/JIP3/MA2K7/JNK pathway in early brain injury after SAH in rats. Neuropharmacology 108:316–323

    Article  CAS  Google Scholar 

  • Zhang F, Si M, Wang H, Mekhemar MK, Dörfer CE, Fawzy El-Sayed KM (2017) IL-1/TNF-α inflammatory and anti-inflammatory synchronization affects gingival stem/progenitor cells’ regenerative attributes. Stem Cells Int. 2017:1349481. https://doi.org/10.1155/2017/1349481

  • Zhang D, Yu YJ, Xu FS, Yuan JH, Wang R, Zhang CS, Wang LX, Liu Y, Song LM, Liu JL, Dong J (2019) Recombinant betatrophin (Angptl8/lipasin) ameliorates streptozotocininduced hyperglycemia and βcell destruction in neonatal rats. Mol Med Rep (5):4523–4532

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Authors and Affiliations

  1. Zoology Department, Faculty of Science, Cairo University, Giza, Egypt

    Atef Abdel-Moneem Ali

  2. Chemistry Department, Faculty of Science, Helwan University, Helwan, Egypt

    Ehab Abdel-Raouf Essawy

  3. Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

    Heba Salah El-Din Fathy Hamed & Fawzy Ali Attaby

  4. Zoology and Entomology Department, Faculty of Science, Helwan University, Helwan, Egypt

    Ahmed E. Abdel Moneim

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  1. Atef Abdel-Moneem Ali
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  5. Fawzy Ali Attaby
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Contributions

Atef Abdel-Moneem Ali contributed to the experimental design, statistical analysis, manuscript writing and editing. Ehab Abdel-Raouf Essawy contributed to the experimental design and experimental procedures. Heba Salah El-Din Fathy Hamed contributed to the animal handling and biochemical analysis. Ahmed E. Abdel Moneim contributed to the molecular analysis and experimental procedures. Fawzy Ali Attaby contributed to the experimental design and manuscript editing.

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Correspondence to Atef Abdel-Moneem Ali.

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The applied procedures and techniques, in the present study, were approved by Cairo University - Institutional Animal Care and Use Committee (CU-IACUC), Giza, Egypt. The approval number is CU/I/F/21/19. Handling of the animals was performed according to the international guidelines of the laboratory animal care and use.

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Ali, A.AM., Essawy, E.AR., Hamed, H.S.ED.F. et al. The ameliorative role of Physalis pubescens L. against neurological impairment associated with streptozotocin induced diabetes in rats. Metab Brain Dis 36, 1191–1200 (2021). https://doi.org/10.1007/s11011-021-00730-7

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