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Bone morphogenetic protein-2 and pulsed electrical stimulation synergistically promoted osteogenic differentiation on MC-3T3-E1 cells

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Abstract

Electrical stimulation (ES) plays an important role in regulating cell osteoblast differentiation. As a noninvasive rehabilitation therapy method, Es has a unique role in postoperative recovery. Bone morphogenetic protein-2 (BMP-2) is the most commonly used bioactive molecule in in situ tissue engineering scaffolds, and it plays an important regulatory role in the whole process of bone injury repair. In this study, the osteogenic regulation of MC-3T3-E1 cells was studied by combining pulsed electrical stimulation (PES) and different concentrations of BMP-2. The results showed that PES and BMP-2 could synergically promote the proliferation of MC-3T3-E1 cells. The qPCR results of osteoblast-related genes showed that PES was synergistic with BMP-2 to promote osteoblast differentiation mainly through the regulation of the Smad/BMP and insulin like growth factor 1 (IGF1) signaling pathways. The expression level of alkaline phosphatase (ALP) and alizarin red staining further demonstrated the synergistic effect of PES and BMP-2 on promoting osteogenic differentiation and mineralization of cells. PES and BMP-2 could also synergically promote cell proliferation, expression of collagen I (COL-I) and ALP, and cell mineralization on the 3D-printed polylactic acid scaffold. These results suggest that the use of PES can enhance the osteogenic effect of in situ bone repair scaffolds containing BMP-2, reduce the dose of BMP-2 alone, and reduce the possible side effects of high-dose BMP-2 in vivo.

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Funding

This work was supported by the Dengfeng Project of Foshan Hospital of Traditional Chinese Medicine (202100043) and the Jilin Scientific and Technological Development Program (20200404110YY). Science and Technology Cooperation Project between Jilin Province and Chinese Academy of Science (No.2022SYHZ0022).

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Authors and Affiliations

  1. Department of Rehabilitation Medicine, Foshan Hospital of Traditional Chinese Medicine, Foshan, 528000, People’s Republic of China

    Shaodong Xie, Deming Zeng, Hanwen Luo, Ping Zhong & Zhiqiang Xu

  2. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, People’s Republic of China

    Yu Wang & Peibiao Zhang

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  1. Shaodong Xie
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Contributions

SX, ZX, PZ and YW designed and conceived this study. SX and DZ carried out the experimental work and wrote the manuscript. HL and PZ carried out in vitro cell experiments. ZX and YW supervised the study and revised the manuscript. The author(s) read and approved the final manuscript.

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Correspondence to Yu Wang or Zhiqiang Xu.

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Xie, S., Zeng, D., Luo, H. et al. Bone morphogenetic protein-2 and pulsed electrical stimulation synergistically promoted osteogenic differentiation on MC-3T3-E1 cells. Mol Cell Biochem (2024). https://doi.org/10.1007/s11010-023-04916-8

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