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Effects of different maternal diets on adipose tissue inflammation and liver tissue oxidative stress in dams and their female offspring

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Abstract

Pregnancy and lactation are important stages of fetal development. Therefore, this study investigated how different maternal diets offered during gestation and lactation periods affect adipose tissue inflammation and liver tissue oxidative stress of dams and their female offspring. Female BALB/c albino mice (60 days old) were randomized into three groups receiving a standard (CONT), hypercaloric (HD), or restricted (RD) diet during the pregnancy. After birth, female offspring weaned at 21 days were divided into two groups that received a standard or restricted diet (CONT/CONT, CONT/RD, RD/CONT, RD/RD, HD/CONT, and HD/RD) until 100 days old. Histological, oxidative parameters and inflammatory infiltrate of dams’ and offspring’s liver and adipose tissue were evaluated. HD dams presented non-alcoholic steatohepatitis (NASH) diagnosis and an increase in tumor necrosis factor-alpha (TNF-α) concentrations when compared to the RD and CONT dams, indicating a pro-inflammatory state. High concentrations of malondialdehyde (MDA) formation and catalase (CAT) activity in HD when compared to the CONT in the liver. SOD activity decreased in RD mice compared to CONT, and the SOD/CAT ratio was decreased in the RD and HD in comparison to the CONT. The maternal diet leads to an increase in SOD in RD/RD compared to HD/RD. RD-fed dams showed an increase in inflammatory infiltrates compared to CONT, evidencing changes caused by a restrictive diet. In the HD/CONT offspring, we verified an increase in inflammatory infiltrates in relation to the offspring fed a standard diet. In conclusion, HD, and RD, during pregnancy and lactation, altered the liver and adipose tissues of mothers. Furthermore, the maternal diet negatively impacts the offspring’s adipose tissue but does not cause liver damage in these animals in adult life.

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Funding

This work was supported by CAPES/Brazil and PROAP/UFCSPA.

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Authors and Affiliations

  1. Graduate Program in Health Science, Federal University of Health Sciences of Porto Alegre (UFCSPA), Porto Alegre, 90050-170, Brazil

    Joana Fisch, Silvana Almeida, Renata Padilha Guedes, Alethéa Gatto Barschak & Márcia Giovenardi

  2. Graduate Program in Biosciences, Federal University of Health Sciences of Porto Alegre (UFCSPA), Porto Alegre, 90050-170, Brazil

    Ana Carolina de Moura, Vanessa Feistauer, Luiza Steffens Reinhardt, Patrícia Molz, Ana Moira Morás, Dinara Jaqueline Moura, Priscila Oliveira de Souza, Elizandra Braganhol, Silvana Almeida, Renata Padilha Guedes & Márcia Giovenardi

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Contributions

JF, AGB and MG conceived and designed the study. JF, ACM, VF, LSR, AMM, and POS performed the experiment. RPG, AGB and MG analyzed and interpreted all the data. All authors were involved in drafting and revising the manuscript.

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Correspondence to Márcia Giovenardi.

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Fisch, J., de Moura, A.C., Feistauer, V. et al. Effects of different maternal diets on adipose tissue inflammation and liver tissue oxidative stress in dams and their female offspring. Mol Cell Biochem (2023). https://doi.org/10.1007/s11010-023-04791-3

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