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Rutin ameliorates metabolic acidosis and fibrosis in alloxan induced diabetic nephropathy and cardiomyopathy in experimental rats

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Abstract

Diabetic nephropathy and cardiomyopathy are two major causes of mortality among patients with diabetes mellitus (DM). Since current diabetic medications are associated with various side effects, the naturally occurring plant-derived compounds are in demand. Bioflavonoids originating from vegetables and medicinal plants have beneficial effects on diabetes by improving glycemic control, lipid metabolism, and anti-oxidant status. The present study is focused on the effect of rutin against alloxan induced diabetic nephropathy and cardiomyopathy. Male albino Wistar rats were divided into four groups, each of six rats. Group I control rats received 0.9% saline as a single dose intraperitoneally. Group II rats were induced diabetes with a single dose of alloxan monohydrate (150 mg/kg body weight in 0.9% saline) intraperitoneally. Group III rats received 0.28 M of NH4Cl in drinking water for 3 days for the experimental induction of metabolic acidosis. Group IV rats were injected with a single dose of alloxan monohydrate (150 mg/kg bodyweight) and administered rutin hydrate (100 mg/kg) for a period of 4 weeks by oral gavage. Administration of rutin prevented urinary ketone body formation and decreased serum creatinine and urea levels in alloxan induced diabetic rats. Rutin supplementation reduced the levels of serum triglycerides and cholesterol in diabetic rats. Gene expression profiling of metabolic acidosis related genes (AQP2, AQP3 and V2R) and also histopathological results demonstrated the protective effect of rutin against diabetic ketoacidodis and fibrosis. The results of the present study revealed rutin administration prevents the progression of diabetic nephropathy and cardiomyopathy through amelioration of fibrosis and metabolic acidosis.

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Acknowledgments

This work was supported by DBT-IPLS, CSIR-SRF fellowship, New Delhi, India. The authors also thank UGC-CEGS, UGC-CAS, UGC-NRCBS, DST-FIST, and DST-PURSE program for the central instrumentation facility at SBS, MKU.

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Authors and Affiliations

  1. Molecular Cardiology Unit, Department of Biochemistry, Center for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, 625 021, India

    Divya Ganesan, Abhishek Albert, Eldho Paul, Karthikeyan Ananthapadmanabhan & Selvam Govindan Sadasivam

  2. Government Sivagangai Medical College and Hospital, Sivagangai, Tamil Nadu, 630 561, India

    Rathinavel Andiappan

  3. Department of Biochemistry, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, India

    Selvam Govindan Sadasivam

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  1. Divya Ganesan
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  2. Abhishek Albert
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  5. Rathinavel Andiappan
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Correspondence to Selvam Govindan Sadasivam.

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The experimental procedure was approved by the Internal Research and Review Board, Ethical Clearance, Biosafety and Animal Welfare Committee of Madurai Kamaraj University.

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Ganesan, D., Albert, A., Paul, E. et al. Rutin ameliorates metabolic acidosis and fibrosis in alloxan induced diabetic nephropathy and cardiomyopathy in experimental rats. Mol Cell Biochem 471, 41–50 (2020). https://doi.org/10.1007/s11010-020-03758-y

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  • DOI: https://doi.org/10.1007/s11010-020-03758-y

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