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Cytoglobin inhibits migration through PI3K/AKT/mTOR pathway in fibroblast cells

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Abstract

Cell proliferation and migration are crucial in many physiological processes including development, cancer, tissue repair, and wound healing. Cell migration is regulated by several signaling molecules. Identification of genes related to cell migration is required to understand molecular mechanism of non-healing chronic wounds which is a major concern in clinics. In the current study, the role of cytoglobin (CYGB) gene in fıbroblast cell migration and proliferation was described. L929 mouse fibroblast cells were transduced with lentiviral particles for CYGB and GFP, and analyzed for cell proliferation and migration ability. Fibroblast cells overexpressing CYGB displayed decreased cell proliferation, colony formation capacity, and cell migration. Phosphorylation levels of mTOR and two downstream effectors S6 and 4E-BP1 which take part in PI3K/AKT/mTOR signaling declined in CYGB-overexpressing cells. Microarray analysis indicated that CYGB overexpression leads to downregulation of cell proliferation, migration, and tumor growth associated genes in L929 cell line. This study demonstrated the role of CYGB in fibroblast cell motility and proliferation. CYGB could be a promising candidate for further studies as a potential target for diseases related to cell migration such as cancer and chronic wound treatment.

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Acknowledgements

We would like to thank NIH Fellows editorial board for their assistance for manuscript editing.

Funding

This study was supported by Yeditepe University.

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Authors and Affiliations

  1. Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Kayisdagi Cad. 26 Agustos Yerlesimi, 34755, Atasehir, Istanbul, Turkey

    Selami Demirci, Ayşegül Doğan, Hüseyin Apdik, Emre Can Tuysuz, Sukru Gulluoglu & Fikrettin Şahin

  2. National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD, USA

    Selami Demirci

  3. National Cancer Instıtute, CDBL, NIH, Frederıck, MD, USA

    Ayşegül Doğan

  4. Department of Medical Genetics, Yeditepe University Medical School Inonu Mah, Kayisdagi Cad. 26 Agustos Yerlesimi, 34755, Atasehir, Istanbul, Turkey

    Emre Can Tuysuz, Sukru Gulluoglu & Omer Faruk Bayrak

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  1. Selami Demirci
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Correspondence to Ayşegül Doğan.

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Demirci, S., Doğan, A., Apdik, H. et al. Cytoglobin inhibits migration through PI3K/AKT/mTOR pathway in fibroblast cells. Mol Cell Biochem 437, 133–142 (2018). https://doi.org/10.1007/s11010-017-3101-2

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