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Association of genetic variants of pentraxin 3 rs3816527 with hypertension in Chronic kidney disease patients

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Abstract

Hypertension is a nearly constant feature and both a cause and a consequence of chronic kidney disease (CKD). Atherosclerotic lesions showed a marked expression of pentraxin 3 on the surface of lumen and in the plaque. The aim was to assess the correlation of exon 2 of pentraxin 3 gene SNP rs3816527 with hypertension with CKD. The study was conducted on 110 CKD patients (60 patients with and 50 patients without hypertension) and 40 healthy subjects as control. Laboratory investigations including the measurement of fasting blood glucose, lipid profile, and indices of oxidative stress, liver function tests, and renal function tests were done. Genotyping of pentraxin 3 gene SNP rs3816527 was done by real-time PCR. There was a significant difference between CKD patients with hypertension and the subjects in the control group regarding systolic and diastolic BP, urea, creatinine, GFR, TG, cholesterol, LDL, HDL, and total antioxidant levels (p < 0.001). There is a statistically significant difference between CKD patients with hypertension and the other studied groups regarding the frequencies of AA genotype and A allele of exon 2 SNP of pentraxin 3 gene compared to CC genotype and C allele (wild type) (p < 0.001), while there was significant difference between CKD patients without hypertension and control (p > 0.05). Pentraxin 3 AA genotype SNP rs3816527 can be considered as a potential biomarker and a risk factor for CKD patients, especially hypertensive patients, and specifically as an independent predictor of hypertension in CKD.

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References

  1. Kokubo Y, Nakamura S, Okamura T et al (2009) Relationship between blood pressure category and incidence of stroke and myocardial infarction in an urban Japanese population with and without chronic kidney disease: the SUITA study. Stroke 40:2674–2679

    Article  PubMed  Google Scholar 

  2. Cachofeiro V, Miana M, De Las Heras N et al (2009) Inflammation: a link between hypertension and atherosclerosis. Curr Hypertens Rev 5:40–48

    Article  CAS  Google Scholar 

  3. Stenvinkel P (2010) Chronic kidney disease—a public health priority and harbinger of premature cardiovascular disease. J Intern Med 268:456–467

    Article  CAS  PubMed  Google Scholar 

  4. Coresh J, Selvin E, Stevens LA et al (2010) Prevalence of chronic kidney disease in the united states. JAMA 298:2038–2047

    Article  Google Scholar 

  5. Honda H, Qureshi AR, Axelsson J et al (2007) Obese sarcopenia in patients with end-stage renal disease is associated with inflammation and increased mortality. Am J Clin Nutr 86:633–638

    CAS  PubMed  Google Scholar 

  6. Inforzato A, Jaillon S, Moalli F et al (2011) The long pentraxin PTX3 at the crossroads between innate immunity and tissue remodelling. Tissue Antigens 77(4):271–282

    Article  CAS  PubMed  Google Scholar 

  7. Norata GD, Marchesi P, Pulakazhi Venu VK et al (2009) Deficiency of the long pentraxin ptx3 promotes vascular inflammation and atherosclerosis. Circulation 120(8):699–708

    Article  CAS  PubMed  Google Scholar 

  8. Deban L, Russo RC, Sironi M et al (2010) Regulation of leukocyte recruitment by the long pentraxin PTX3. Nat Immunol 11(4):328–334

    Article  CAS  PubMed  Google Scholar 

  9. Klouche M, Peri G, Knabbe C et al (2004) Modified atherogenic lipoproteins induce expression of pentraxin-3 by human vascular smooth muscle cells. Atherosclerosis 175(2):221–228

    Article  CAS  PubMed  Google Scholar 

  10. Norata GD, Pirillo A, Ammirati E et al (2012) Emerging role of high density lipoproteins as a player in the immune system. Atherosclerosis 220(1):11–21

    Article  CAS  PubMed  Google Scholar 

  11. Wallach JB (ed) (1996) Metabolic and hereditary disorders. In: Interpretation of diagnostic tests. 6th ed. Little Brown and Company: Boston, MA

  12. Larsen K (1972) Creatinine assay by a reaction-kinetic principle. Clin Chim Acta 41:209–217

    Article  CAS  PubMed  Google Scholar 

  13. Talke H, Schubert GE (1965) Enzymatic determination of urea using the coupled urease-GLDH enzyme system. Mediat Inflamm 43:174–176

    CAS  Google Scholar 

  14. Levey AS, Coresh J, Greene T et al (2006) Using standardized serum creatinine values in the modification of diet in renal disease study equation for estimating glomerular filtration rate. Ann Intern Med 145:247–254

    Article  CAS  PubMed  Google Scholar 

  15. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V (2001) Method for the measurment of total antioxidant activity in human fluids. J Clin Pathol 54:356–361

  16. Ohkawa H, Ohishi W, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid. Anal Biochem 95:351

  17. Souza DG, Amaral FA, Fagundes CT (2009) The long pentraxin PTX3 is crucial for tissue inflammation after intestinal ischemia and reperfusion in mice. Am J Pathol 174:1309–1318

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Suliman ME, Qureshi AR, Carrero JJ (2008) The long pentraxin PTX-3 in prevalent hemodialysis patients: associations with comorbidities and mortality. QJM 101:397–405

    Article  CAS  PubMed  Google Scholar 

  19. Witasp A, Ryden M, Carrero JJ, Qureshi AR, Nordfors L, Naslund E et al (2013) Elevated circulating levels and tissue expression of pentraxin 3 in uremia: a reflection of endothelial dysfunction. PLoS ONE 8:e63493

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Barbati E, Specchia C, Villella M et al (2012) Influence of pentraxin3 (PTX3) genetic variants on myocardial infarction risk and PTX3 plasma levels. PLoS ONE 7:e53030

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Breviario F, d’Aniello EM, Golay J et al (1992) Interleukin-1-inducible genes in endothelial cells: cloning of a new gene related to C-reactive protein and serum amyloid P component. J Biol Chem 267:22190–22197

    CAS  PubMed  Google Scholar 

  22. Nauta AJ, Bottazzi B, Mantovani A et al (2003) Biochemical and functional characterization of the interaction between pentraxin 3 and C1q. Eur J Immunol 33:465–473

    Article  CAS  PubMed  Google Scholar 

  23. Abbas MA, Mohamed NY, Abd El-fattah WM et al (2013) Plasma pentraxin-3 level as a biomarker in patients with chronic kidney disease and its association with cardiovascular complications. Life Sci J 10(2):2949–2958

    Google Scholar 

  24. Lech M, Rommele C, Anders HJ (2012) Pentraxins in nephrology: C-reactive protein, serum amyloid P and pentraxin-3. Nephrol Dial Transplant 0:1–8

    Google Scholar 

  25. Argani H, Ghorbanihaghjo A, Panahi G et al (2012) Serum fetuin-A and pentraxin3 in hemodialysis and renal transplant patients. Clin Biochem 45:775–779

    Article  CAS  PubMed  Google Scholar 

  26. Sjöberg B, Qureshi AR, Heimbürger O, Stenvinkel P, Lind L, Larsson A, Bárány P, Ärnlöv J (2016) Association between levels of pentraxin 3 and incidence of chronic kidney disease in the elderly. J Intern Med 279(2):173–179

    Article  PubMed  Google Scholar 

  27. Parlak A, Aydoğan U, İyisoy A et al (2012) Elevated pentraxin-3 levels are related to blood pressure levels in hypertensive patients: an observational study. Anadolu Kardiyol Derg 12(4):298–304

    PubMed  Google Scholar 

  28. Mantovani A, Garlanda C, Bottazzi B et al (2006) The long pentraxin PTX3 in vascular pathology. Vasc Pharmacol 45(5):326–330

    Article  CAS  Google Scholar 

  29. Gürsu M, Aydin Z, Öztürk S (2014) Is pentraxin-3 a stronger marker of inflammation than C-reactive protein in chronic kidney disease? Turk Neph Dial Transpl 23(2):131–136

    Article  Google Scholar 

  30. Methe H, Edelman ER (2006) Cell-matrix contact prevents recognition and damage of endothelial cells in states of heightened immunity. Circulation 114(Suppl 1):1233–1238

    Google Scholar 

  31. Carrizzo A, Lenzi P, Procaccini C, Damato A, Biagioni F, Ambrosio M et al (2015) Pentraxin 3 induces vascular endothelial dysfunction through a P-selectin/matrix metalloproteinase-1 pathway. Circulation 131:1495–1505

    Article  CAS  PubMed  Google Scholar 

  32. Fornai F, Carrizzo A, Forte M et al (2016) The inflammatory protein pentraxin 3 in cardiovascular disease. Immun Ageing 13:25

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Menoufia University, Shebin ElKom, Menoufia, 32511, Egypt

    Eman A. E. Badr, Ghada E. Hamoda & Safaa I. Tayel

  2. Department of Internal Medicine, Faculty of Medicine, Menoufia University, Shebin ElKom, Egypt

    Elsayed I. Elshayeb

Authors
  1. Eman A. E. Badr
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  2. Ghada E. Hamoda
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  3. Safaa I. Tayel
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  4. Elsayed I. Elshayeb
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Correspondence to Safaa I. Tayel.

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The study was conducted in accordance with the Declaration of Helsinki. All participants provided written informed consent, and the Ethics Committee of Faculty of Medicine, Menoufia University approved the study protocol.

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Badr, E.A.E., Hamoda, G.E., Tayel, S.I. et al. Association of genetic variants of pentraxin 3 rs3816527 with hypertension in Chronic kidney disease patients. Mol Cell Biochem 425, 203–212 (2017). https://doi.org/10.1007/s11010-016-2874-z

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  • DOI: https://doi.org/10.1007/s11010-016-2874-z

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