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A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction

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Abstract

The main function of annexin A1 (ANXA1), a member of the annexin superfamily, is to bind to cellular membranes in a Ca2+-dependent manner. In skeletal muscle, ANXA1 is thought to be involved in the repair of damaged membrane tissue and in the migration of muscle cells. We hypothesized that ANXA1 is one of the myokines secreted during muscle contractions to accelerate the repair of cell damage after contraction. Here we performed cell contractions by electric pulse stimulation; the results revealed that a fragmented form of ANXA1 was cleaved by calpain and selectively secreted from skeletal muscle cells by contraction. We therefore realized that muscle-contraction-induced calpain-dependent ANXA1 fragmentation has a wound-healing effect on damaged cells. This suggested that not the intact form but rather fragmented ANXA1 is a contraction-induced myokine.

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Acknowledgments

This work was supported by a grant-in-aid for Scientific Research(C) to N.G.-I. and by a grant-in-aid from the Funding Program for World-Leading Innovative R&D on Science and Technology by the Council for Science and Technology Policy to N.L.F.

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Authors and Affiliations

  1. Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252-0880, Japan

    Naoko Goto-Inoue

  2. Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1, Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan

    Kotaro Tamura, Fumika Motai, Miyuki Ito, Kaede Miyata, Yasuko Manabe & Nobuharu L. Fujii

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  1. Naoko Goto-Inoue
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  2. Kotaro Tamura
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  5. Kaede Miyata
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  7. Nobuharu L. Fujii
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Correspondence to Nobuharu L. Fujii.

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Goto-Inoue, N., Tamura, K., Motai, F. et al. A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction. Mol Cell Biochem 411, 173–180 (2016). https://doi.org/10.1007/s11010-015-2579-8

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  • DOI: https://doi.org/10.1007/s11010-015-2579-8

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