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CCAAT-enhancer binding protein (C/EBP) β regulates insulin-like growth factor (IGF) 1 expression in porcine liver during prenatal and postnatal development

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Abstract

IGF1 expression regulation attracts numerous interests because of its important role during mammalian growth and development. Domestic pig can be used as a valuable animal model to investigate human development since they share the high similarity in general physiology and metabolism. In this study, we examined the expression pattern of IGF1 and found it associated with liver C/EBP β expression pattern in porcine liver during embryonic and postnatal development. Both IGF1 and C/EBP β expression in liver maintained at low levels before birth and increased after birth. Correspondingly, C/EBP β demonstrated high binding activity to two sites at IGF1 promoter region in liver after birth. Additionally, IGF1 expression can be activated by C/EBP β overexpression in porcine primary hepatocytes. These results indicated that C/EBP β can activate IGF1 expression after birth by binding to IGF1 promoter. Our study may contribute to a better understanding of mammalian development and bring a novel anti-aging pathway in human.

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Acknowledgments

This study was supported by Grants from the following Projects: Study on the regulation of IGF1 gene in pig liver (National Natural Science Foundation of China 31472073 2015-2019); Cultivating New Varieties by Transgenic Technology (#2012ZX08006-003); and China Scholarship Council.

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Authors and Affiliations

  1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Yiting Tang, Kai Xiong, Ming Shen & Honglin Liu

  2. Department of Gene and Cell Engineering, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100094, People’s Republic of China

    Yiting Tang, Yulian Mu & Kui Li

  3. Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Grønnegårdsvej 7, 1870, Frederiksberg C, Denmark

    Kai Xiong

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  1. Yiting Tang
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Correspondence to Honglin Liu.

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DNA size range analysis by agrose gel electrophoresis after 5min and 10min of sonication. Below is the link to the electronic supplementary material.

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Tang, Y., Xiong, K., Shen, M. et al. CCAAT-enhancer binding protein (C/EBP) β regulates insulin-like growth factor (IGF) 1 expression in porcine liver during prenatal and postnatal development. Mol Cell Biochem 401, 209–218 (2015). https://doi.org/10.1007/s11010-014-2308-8

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  • DOI: https://doi.org/10.1007/s11010-014-2308-8

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