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Curcumin causes promoter hypomethylation and increased expression of FANCF gene in SiHa cell line

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Abstract

Curcumin and resveratrol were evaluated for their potential to cause reversal of promoter hypermethylation and associated gene expression of FANCF in SiHa cell line. Methylation specific PCR along with bisulphite sequencing revealed the demethylation of 12 CpG sites out of 15 CpG sites spanning +280 to +432 region of FANCF promoter after treatment with curcumin and fivefold up regulation of FANCF gene expression as shown by qRT-PCR. In vitro methylation assay also showed that M.SssI an analogue of DNMT1 was effectively inhibited at 50 μM concentration of curcumin. Resveratrol was not found to be effective in causing reversal of promoter hypermethylation of FANCF gene when used at 20 μM for 4 days in SiHa cell line.

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Acknowledgments

This study was supported by Council of Scientific and Industrial Research (CSIR), New Delhi, India.

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The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Department of Biotechnology, Panjab University, Sector 14, Chandigarh, 160014, India

    Gaurav Parashar & Neena Capalash

  2. Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, India

    Nidarshana Chaturvedi Parashar

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  1. Gaurav Parashar
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  2. Nidarshana Chaturvedi Parashar
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  3. Neena Capalash
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Correspondence to Neena Capalash.

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Parashar, G., Parashar, N.C. & Capalash, N. Curcumin causes promoter hypomethylation and increased expression of FANCF gene in SiHa cell line. Mol Cell Biochem 365, 29–35 (2012). https://doi.org/10.1007/s11010-012-1240-z

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  • DOI: https://doi.org/10.1007/s11010-012-1240-z

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