Abstract
Human Fibroblast growth factor 1 (FGF1) has been recognized as a valuable protein drug for the treatment of many diseases because of its multiple functions in regulating a variety of biological processes involved in embryonic development, cell growth and differentiation, morphogenesis, tissue repair, and others. The aim of this study was to develop an FGF1 mammary gland-specific expression vector to produce FGF1 on a large scale from transgenic cows to meet the demand for FGF1 in medical use. In this study, we generated an FGF1 mammary gland-specific expression vector and validated its function in human MCF-7 cells. This vector was shown to successfully express functional FGF1, thus potentially enabling the generation of transgenic cows to be used as mammary gland bioreactors.
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Acknowledgments
This study was supported by a grant from the China National Foundation for Natural Science (13002104) to HO. All vectors constructed in this article are available from the authors upon request from academic researchers.
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Yang Zhou and Linzhu Ren contributed equally to this study.
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Zhou, Y., Ren, L., Zhu, J. et al. Construction of a recombinant human FGF1 expression vector for mammary gland-specific expression in human breast cancer cells. Mol Cell Biochem 354, 39–46 (2011). https://doi.org/10.1007/s11010-011-0803-8
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DOI: https://doi.org/10.1007/s11010-011-0803-8