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Role of cyclooxygenase-2 functional gene polymorphisms in Helicobacter pylori induced gastritis and gastric atrophy

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Abstract

In India, the role of host genetic factors is poorly studied for Helicobacter pylori associated diseases. Therefore, we evaluated the association of functionally relevant COX-2 gene polymorphisms (−765 G>C and +8473 T>C) in gastritis and precancerous lesions susceptibility. After upper GI endoscopy, 130 rapid urease test positive patients with non-ulcer dyspepsia, also showed positivity for H. pylori using modified Geimsa staining and anti-CagA IgG serology were included. All patients and 260 asymptomatic controls were genotyped for COX-2 variations using PCR-RFLP. COX-2 −765 (GC+CC) genotypes, −765 C allele, +8473 CC genotype, +8473 (TC+CC) genotypes, +8473 C allele, and variant haplotypes imparted high risk for gastritis (P = 0.036, OR = 1.82; P = 0.007, 1.92; P = 0.025, OR = 2.13; P = 0.017, OR = 1.80; P = 0.017, OR = 1.45; P = 0.010, OR = 2.40; P = 0.023, OR = 1.50 and P = 0.012, OR = 2.20 folds, respectively). In contrast, COX-2 −765 C allele carriers had low risk for lymphocyte (P = 0.020, OR = 0.35), plasma cell infiltrations (P = 0.016, OR = 0.33), and gastric atrophy (GA) development (P = 0.019, OR = 0.35). In conclusion, COX-2 variant allele/genotype/haplotype carriers may be at high risk for gastritis. However, COX-2 −765 C allele carriers may be at low risk for GA development.

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Acknowledgments

Council of Scientific and Industrial Research (CSIR) New Delhi, India is greatly acknowledged for providing Junior and Senior Research fellowship to Mr. B. R. Achyut.

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Authors and Affiliations

  1. Department of Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226014, UP, India

    B. R. Achyut, Nikhil Moorchung & Balraj Mittal

  2. Department of Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226014, UP, India

    Uday C. Ghoshal

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  1. B. R. Achyut
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Correspondence to Balraj Mittal.

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Achyut, B.R., Ghoshal, U.C., Moorchung, N. et al. Role of cyclooxygenase-2 functional gene polymorphisms in Helicobacter pylori induced gastritis and gastric atrophy. Mol Cell Biochem 321, 103–109 (2009). https://doi.org/10.1007/s11010-008-9924-0

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