Abstract
In tumor cells, alterations in cellular glycosylation may play a key role in their metastatic behavior. Using small interfering RNA against GnT-V, we found that the expression of GnT-V and β1,6GlcNAc branching were significantly reduced which was particularly accompanied by the arrest in both cell migration and invasion as compared to the negative control. Moreover, the suppressed GnT-V expression by siRNA technique inactivated the signaling molecules including Rac1, cofilin, Erk and Akt, and activated RhoA levels in cells lacking GnT-V, but revealed no impact on Cdc42 activity. All these notions disclose for the first time that GnT-V and β1, 6GlcNAc branching mediate the cell migration and invasion in Rac1-positive and RhoA-negative regulatory manners.
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Abbreviations
- FBS:
-
Fetal bovine serum
- FITC:
-
Fluorescein isothyocianate
- SDS-PAGE:
-
Sodium dodecyl sulfate polyacrylamide gel electrophoresis
- mAb:
-
Monoclonal antibody
- HRP:
-
Horseradish peroxidase
- PBS:
-
Phosphate-buffered saline
- BSA:
-
Bovine serum albumin
- TBS:
-
Tris-buffered saline
- PHA-L:
-
Phaseolus vulgaris Leucoagglutinin
- Fn:
-
Fibronectin
- GnT-V:
-
N-acetylglucosaminyltransferase V
- PI3K:
-
Phosphatidylinositol 3-kinase
- MAPK:
-
Mitogen-activated protein kinase
- Erk:
-
Extracellular signal-regulated kinase
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Acknowledgment
This research was supported by the National Basic Research Program Grant (973) (2003CB716400).
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Yunxue Zhao and Jing Li contributed equally to this work.
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Zhao, Y., Li, J., Xing, Y. et al. N-acetylglucosaminyltransferase V mediates cell migration and invasion of mouse mammary tumor cells 4TO7 via RhoA and Rac1 signaling pathway . Mol Cell Biochem 309, 199–208 (2008). https://doi.org/10.1007/s11010-007-9656-6
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DOI: https://doi.org/10.1007/s11010-007-9656-6