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Alteration of mitochondrial oxidative capacity during porcine preadipocyte differentiation and in response to leptin

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Abstract

Mitochondrial apparatus is a fundamental aspect in cell, serving for amino acid biosynthesis, fatty acid oxidation (FAO), and ATP production. In this article, we investigated the change of mitochondrial oxidative capacity during porcine adipocyte differentiation and in response to leptin. Rhodamine 123 staining analysis showed about 2-fold increase of mitochondrial membrane electric potential in differentiated adipocyte in comparison with preadipocyte. The mRNA expression of Cytochromes c (Cyt c), carnitine palmitoyltransferase 1 (CPT1), and malate dehydrogenases (MDH) increased markedly (P < 0.05), but that of UCP2 decreased (P < 0.05). Moreover PGC-1α and UCP3 was very low and showed no changes during the adipocyte differentiation. The protein expression of Cyt c and the enzyme activity of Cytochrome c oxidase (COX) increased with preadipocyte differentiation, but cellular ATP level decreased. Furthermore, at the level of 10 and 100 ng/ml leptin not only selectively increased the gene expression of PGC-1α, CPT1, Cyt c, UCP2, and UCP3 (P < 0.05), but also enhanced COX enzyme activity which related to mitochondrial FAO. There is no change of Mitochondrial membrane electric potential and ATP level in cell treated by leptin. These results suggested Mitochondrial is not only critical in FAO, but also play an important role in adipogenesis.

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Abbreviations

Cyt c :

Cytochromes c

CEBP/α:

CAAT/enhancer binding protein α

PPAR:

Peroxisome proliferator activated receptor

PGC-1:

Peroxisome proliferator-activated receptor γ coactivator

COX:

Cytochrome c oxidase

CPT1:

Carnitine palmitoyltransferase 1

MDH:

Malate dehydrogenases

UCP:

Uncoupling protein

MRC:

Mitochondrial respiratory chain

NRF:

Nuclear respiratory factors

mtTFA:

Mitochondrial transcriptional factor A

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Acknowledgments

This work was supported by the National Basic Research Program of China under Grant number 2004CB117506 and National Natural Science Foundation under Grant number 30471239.

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Authors and Affiliations

  1. Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China

    Gui-Fen Luo, Tai-Yong Yu, Xu-Hui Wen, Ying Li & Gong-She Yang

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  1. Gui-Fen Luo
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  2. Tai-Yong Yu
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  4. Ying Li
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  5. Gong-She Yang
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Correspondence to Gong-She Yang.

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Luo, GF., Yu, TY., Wen, XH. et al. Alteration of mitochondrial oxidative capacity during porcine preadipocyte differentiation and in response to leptin. Mol Cell Biochem 307, 83–91 (2008). https://doi.org/10.1007/s11010-007-9587-2

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  • DOI: https://doi.org/10.1007/s11010-007-9587-2

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