Abstract
PCR was used to amplify DNA fragments containing the genes for interferon γ (IFNγ)-binding proteins (IFNγBPs) of the variola virus (VARV) and monkeypox virus (MPXV). The genes were expressed from baculovirus DNAs in Sf21 insect cells. The recombinant proteins were isolated from the culture medium by affinity chromatography. PAGE and Western blot analysis of the culture media and affinity-purified recombinant proteins showed that, in contrast to their cell analogs, the viral IFNγBPs form dimers in the absence of the ligand, human IFNγ. The biological activity of recombinant IFNγBPs was inferred from suppression of the protective effect of human IFNγ on L68 cells infected with the mouse encephalomyocarditis virus. The viral proteins showed a dose-dependent IFNγ-neutralizing effect. The prospects of using IFNγBPs as IFNγ antagonists are discussed.
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Translated from Molekulyarnaya Biologiya, Vol. 39, No. 6, 2005, pp. 1055–1062.
Original Russian Text Copyright © 2005 by Nepomnyashchikh, Lebedev, Ryazankin, Pozdnyakov, Gileva, Shchelkunov.
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Nepomnyashchikh, T.S., Lebedev, L.R., Ryazankin, I.A. et al. Comparison of the Interferon γ-Binding Proteins of the Variola and Monkeypox Viruses. Mol Biol 39, 926–933 (2005). https://doi.org/10.1007/s11008-005-0114-9
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DOI: https://doi.org/10.1007/s11008-005-0114-9