Abstract
Type 2 diabetes mellitus (T2DM) is a multifactorial disease that requires multiple therapeutic strategies for its management. Bioactive peptides with multiple anti-diabetic targets are attractive therapeutic molecules. The present study was conducted to identify additional anti-diabetic targets of α-glucosidase inhibitory peptides, SVPA, SEPA, STYV, and STY. The α-glucosidase inhibitory activity of the peptides was in the order STYV > STY > SEPA > SVPA while molecular docking against human dipeptidyl peptidase IV (DPP-IV) showed that SVPA had the best binding affinity. In contrast, in vitro studies indicated that SEPA had a significantly higher (P < 0.05) DPP-IV inhibitory activity (IC50 = 5.78 ± 0.19 mM) than other peptides. SVPA and SEPA showed mixed inhibition pattern while STYV and STY were uncompetitive inhibitors of the enzyme. IPI (diprotin A), STYV and STY were not cytotoxic while SEPA displayed some cytotoxicity. In differentiated 3T3-L1 adipocytes, SVPA and STYV were found to induce a significant (P < 0.05) decrease in intracytoplasmic lipid accumulation when added during the differentiation process while STY, GSH and IPI caused significant reduction (P < 0.05) in the lipid accumulation when added after the differentiation. The SVPA, SEPA and STYV were better scavengers of methylglyoxal than STY but STYV had the best scavenging activities toward reactive oxygen species and nitric oxide. It was concluded that the four α-glucosidase inhibitory peptides including IPI have multiple targets against type T2DM but, overall, of the four peptides evaluated, STYV tends to have better potential for application as a multifunctional anti-diabetic peptide.
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Agyei D, Tsopmo A, Udenigwe CC (2018) Bioinformatics and peptidomics approaches to the discovery and analysis of food-derived bioactive peptides. Anal Bioanal Chem 410:3463–3472. https://doi.org/10.1007/s00216-018-0974-1
Byun HG, Lee JK, Park HG, Jeon JK, Kim SK (2009) Antioxidant peptides isolated from the marine rotifer, Brachionus rotundiformis. Process Biochem 44:842–846. https://doi.org/10.1016/j.procbio.2009.04.003
Cao MM, Lu X, Liu GD, Su Y, Li YB, Zhou J (2018) Resveratrol attenuates type 2 diabetes mellitus by mediating mitochondrial biogenesis and lipid metabolism via Sirtuin type 1. Exp Ther Med 15:576–584. https://doi.org/10.3892/etm.2017.5400
De Fronzo RA, Triplitt CL, Abdul-Ghani M, Cersosimo E (2014) Novel agents for the treatment of type 2 diabetes. Diabetes Spectrum 27:100–112. https://doi.org/10.2337/diaspect.27.2.100
Dhar A, Desai KM, Wu L (2010) Alagebrium attenuates acute methylglyoxal-induced glucose intolerance in Sprague-Dawley rats. Br J Pharmacol 159:166–175. https://doi.org/10.1111/j.1476-5381.2009.00469.x
Eid HM, Thong F, Nachar A, Pierre SH (2017) Caffeic acid methyl and ethyl esters exert potential antidiabetic effects on glucose and lipid metabolism in cultured murine insulin- sensitive cells through mechanisms implicating activation of AMPK. Pharm Biol 55:2026–2034. https://doi.org/10.1080/13880209.2017.1345952
Giacco F, Brownlee M (2010) Oxidative stress and diabetic complications. Circ Res 107:1058–1070. https://doi.org/10.1161/CIRCRESAHA.110.223545
Giustarini D, Rossi R, Milzani A, Dalle-Donne I (2008) Nitrite and nitrate measurement by Griess reagent in human plasma: evaluation of interferences and standardization. Methods Enzymol 440:361–380. https://doi.org/10.1016/S0076-6879(07)00823-3
Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE (2014) Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Res Clin Pract 103:137–149. https://doi.org/10.1016/j.diabres.2013.11.002
Hadi HAR, Suwaidi JA (2007) Endothelial dysfunction in diabetes mellitus. Vasc Health Risk Manag 3:853–876
Hasan MM, Ahmed QU, Soad SZM, Latip J, Taher M, Syafiq TMF, Sarian MN, Alhassan AM, Zakaria ZA (2017) Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cells. BMC Complement Altern Med 17:431. https://doi.org/10.1186/s12906-017-1929-3
Ibrahim MA, Koorbanally N, Islam MS (2014) Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes (α-glucosidase and α-amylase) by Khaya senegalensis. Acta Pharm 64:311–324. https://doi.org/10.2478/acph-2014-0025
Ibrahim MA, Bester MJ, Neitz AWH, Gaspar ARM (2018a) Structural properties of bioactive peptides with α-glucosidase inhibitory activity. Chem Biol Drug Des 91:370–379. https://doi.org/10.1111/cbdd.13105
Ibrahim MA, Bester MJ, Neitz AWH, Gaspar ARM (2018b) Rational in silico design of α- glucosidase inhibitory peptides and in vitro evaluation of promising candidates. Biomed Pharm 107:234–242. https://doi.org/10.1016/j.biopha.2018.07.163
Inzucchi SE, Bergenstal RM, Buse JB, Diamant M, Ferrannini E, Nauck M, Peters AL, Tsapas A, Wender R, Matthews DR (2015) Management of hyperglycaemia in type 2 diabetes, 2015: a patient-centred approach. Update to a position statement of the American Diabetes Association and the European Association for the Study of Diabetes. Diabetologia 58:429–442. https://doi.org/10.2337/dc14-2441
Konrad B, Dabrowski A, Szoltysik M, Marta P, Aleksandra Z, Jozefa C (2014) The evaluation of dipeptidyl peptidase (DPP)-IV, α-glucosidase and angiotensin converting enzyme (ACE) inhibitory activities of whey proteins hydrolysed with serine protease isolated from Asian pumpkin (Cucurbita ficifolia). Int J Pept Res Ther 20:483–491. https://doi.org/10.1007/s10989-014-9413-0
Korhonen H, Pihlanto A (2007) Food-derived bioactive peptides-opportunities for designing future foods. Curr Pharm Des 9:1297–1308. https://doi.org/10.2174/1381612033454892
Lacroix IM, Li-chan EC (2016) Food-derived dipeptidyl-peptidase IV inhibitors as potential approach for glycemic regulation–Current knowledge and future research considerations. Trends Food Sci Technol 54:1–16. https://doi.org/10.1016/j.tifs.2016.05.008
Lafarga T, Connor PO, Hayes M (2014) Identification of novel dipeptidyl peptidase-IV and angiotensin-I-converting enzyme inhibitory peptides from meat proteins using in silico analysis. Peptides 59:53–62. https://doi.org/10.1016/j.peptides.2014.07.005
Lee H, Lee YJ, Choi H, Ko EH, Kim J (2009) Reactive oxygen species facilitate adipocyte differentiation by accelerating mitotic clonal expansion. J Biol Chem 280:10601–10609. https://doi.org/10.1074/jbc.M808742200
Li W, Xu H, Hu Y, He P, Ni Z, Xu H, Zhang Z, Dai H (2013) Edaravone protected human brain microvascular endothelial cells from methylglyoxal-induced injury by inhibiting AGEs/RAGE/oxidative stress. PLoS One 8:e76025. https://doi.org/10.1371/journal.pone.0076025
Li Q, Zhang C, Chen H, Xue J, Guo X, Liang M, Chen M (2018) BioPepDB: an integrated data platform for food-derived bioactive peptides. Int J Food Sci Nutr 69:963–968. https://doi.org/10.1080/09637486.2018.1446916
Matafome P, Rodrigues T, Sena C, Seiça R (2017) Methylglyoxal in metabolic disorders: facts, myths, and promises. Med Res Rev 37:368–403. https://doi.org/10.1002/med.21410
Mojica L, de Majia MG (2016) Optimization of enzymatic production of anti-diabetic peptides from black bean (Phaseolus vulgaris L.) proteins, their characterization and biological potential. Food Funct 7:713–727. https://doi.org/10.1039/C5FO01204J
Mulvihill EE (2018) Dipeptidyl peptidase inhibitor therapy in type 2 diabetes: control of incretin axis and regulation of glucose and lipid metabolism. Peptides 100:158–164. https://doi.org/10.1016/j.peptides.2017.11.023
Nongonierma AB, FitzGerald RJ (2016) Structure activity relationship modelling of milk protein-derived peptides with dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Peptides 79:1–7. https://doi.org/10.1016/j.peptides.2016.03.005
Nongonierma AB, Mooney C, Shields DC, FitzGerald RJ (2013) Inhibition of dipeptidylpeptidase IV and xanthine oxidase by amino acids and dipeptides. Food Chem 141:644–653. https://doi.org/10.1016/j.foodchem.2013.02.115
Nongonierma AB, Mooney C, Shields DC, FitzGerald RJ (2014) In silico approaches to predict the potential of milk protein-derived peptides as dipeptidyl peptidase IV (DPP-IV) inhibitors. Peptides 54:43–51. https://doi.org/10.1016/j.peptides.2014.04.018
Ou B, Huang D, Hampsch-Woodill M, Flanagan JA, Deemer EK (2002) Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: a comparative study. J Agric Food Chem 50:3122–3128. https://doi.org/10.1021/jf0116606
Park YW, Nam MS (2015) Bioactive peptides in milk and dairy products: a review. Korean J Food Sci Anim Res 35:831–840. https://doi.org/10.5851/kosfa.2015.35.6.831
Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE (2004) UCSF Chimera—a visualization system for exploratory research and analysis. J Comput Chem 25:1605–1612. https://doi.org/10.1002/jcc.20084
Rodrigues T, Matafome P, Sereno J, Almeida J, Castelhano J, Gamas L, Neves C, Gonçalves S, Carvalho C, Arslanagic A, Wilcken E (2017) Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698. https://doi.org/10.1038/s41598-017-01730-3
Saito T, Abe D, Sekiya K (2007) Nobiletin enhances differentiation and lipolysis of 3T3-L1 adipocytes. Biochem Biophys Res Commun 357:371–376. https://doi.org/10.1016/j.bbrc.2007.03.169
Semaan DG, Igoli JO, Young L, Gray AI, Rowan EG, Marrero E (2018) In vitro anti-diabetic effect of flavonoids and pheophytins from Allophylus cominia Sw. on the glucose uptake assays by Hep G2, L6, 3T3-L1 and fat accumulation in 3T3-L1 adipocytes. J Ethnopharmacol 216:8–17. https://doi.org/10.1016/j.jep.2018.01.014
Shai LJ, Masoko P, Mokgotho MP, Magano SP, Mogale AM, Boaduo N, Eloff JN (2010) Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa, South Africa. South Afr J Bot 76:465–470. https://doi.org/10.1016/j.sajb.2010.03.002
Siddiqui MA, Rasheed S, Saquib Q, Al-Khedhairy AA, Al-Said MS, Musarrat J, Choudhary MI (2016) In vitro dual inhibition of protein glycation, and oxidation by some Arabian plants. BMC Complement Altern Med 16:276. https://doi.org/10.1186/s12906-016-1225-7
Singh B, Kaur A (2015) Antidiabetic potential of a peptide isolated from an endophytic Aspergillus awamori. J Appl Microbiol 120:301–311. https://doi.org/10.1111/jam.12998
Tiwari N, Thakur AK, Kumar V, Dey A, Kumar V (2014) Therapeutic targets for diabetes mellitus: an update. Clin Pharmacol Biopharm 3:117. https://doi.org/10.4172/2167-065X.1000117
Trott O, Olson AJ (2010) AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading. J Comp Chem 31:455–461. https://doi.org/10.1002/jcc.21334
Udenigwe CC (2014) Bioinformatics approaches, prospects and challenges of food bioactive peptide research. Trends Food Sci Technol 36:137–143. https://doi.org/10.1016/j.tifs.2014.02.004
Usmani SS, Bedi G, Samuel JS, Singh S, Kalra S, Kumar P, Ahuja AA, Sharma M, Gautam A, Raghava GP (2017) THPdb: Database of FDA-approved peptide and protein therapeutics. PLoS ONE 12(7):e0181748. https://doi.org/10.1371/journal.pone.0181748
Varga ZV, Giricz Z, Liaudet L, Haskó G, Ferdinandy P, Pacher P (2015) Interplay of oxidative, nitrosative/nitrative stress, inflammation, cell death and autophagy in diabetic cardiomyopathy. Biochim Biophys Acta 1852:232–242. https://doi.org/10.1016/j.bbadis.2014.06.030
Vulesevic B, McNeill B, Giacco F, Maeda K, Blackburn NJ, Brownlee M, Milne RW, Suuronen EJ (2016) Methylglyoxal-induced endothelial cell loss and inflammation contribute to the development of diabetic cardiomyopathy. Diabetes 65:1699–1713. https://doi.org/10.2337/db15-0568
Wang S, Zeng X, Yang Q, Qiao S (2016) Antimicrobial peptides as potential alternatives to antibiotics in food and animal industry. Int J Mol Sci 17:603. https://doi.org/10.3390/ijms17050603
Wu D, Wang J, Wang H, Ji A, Li Y (2017) Protective roles of bioactive peptides during ischemia-reperfusion injury: from bench to bedside. Life Sci 180:83–92. https://doi.org/10.1016/j.lfs.2017.05.014
Yin CM, Wong JH, Xia J, Ng TB (2013) Studies on anticancer activities of lactoferrin and lactoferricin. Curr Prot Pept Sci 14:492–503. https://doi.org/10.2174/13892037113149990066
Zhang Y, Wang N, Wang W, Wang J, Zhu Z, Li X (2016) Molecular mechanisms of novel peptides from silkworm pupae that inhibit α-glucosidase. Peptides 76:45–50. https://doi.org/10.1016/j.peptides.2015.12.004
Zilleßen P, Celner J, Kretschmann A, Pfeifer A, Racke K, Mayer P (2016) Metabolic role of dipeptidyl peptidase 4 (DPP4) in primary human (pre)adipocytes. Sci Rep 6:23074. https://doi.org/10.1038/srep23074
Acknowledgements
We acknowledge the National Research Foundation of South Africa and the University of Pretoria for financial support. The first author also acknowledges the University of Pretoria for the award of a postdoctoral fellowship position in Biochemistry and Ahmadu Bello University, Zaria, Nigeria for the award of a study fellowship.
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The study was funded by the National Research Foundation of South Africa (Grant Number 91052).
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Mohammed Auwal Ibrahim, June Serem, Megan Bester, Albert Neitz and Anabella Gaspar have declared that they have no conflict of interest.
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Ibrahim, M.A., Serem, J.C., Bester, M.J. et al. New Antidiabetic Targets of α-Glucosidase Inhibitory Peptides, SVPA, SEPA, STYV and STY: Inhibitory Effects on Dipeptidyl Peptidase-IV and Lipid Accumulation in 3T3-L1 Differentiated Adipocytes with Scavenging Activities Against Methylglyoxal and Reactive Oxygen Species. Int J Pept Res Ther 26, 1949–1963 (2020). https://doi.org/10.1007/s10989-019-09993-2
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DOI: https://doi.org/10.1007/s10989-019-09993-2