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Application of microcalorimetry and chemometric analysis

Effects evaluation of angle and nail animal drugs on Bacillus subtilis growth

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Abstract

In this study, microcalorimetry combined with chemometric analysis was used to investigate the effects of angle and nail animal valuable drugs on Bacillus subtilis (B. subtilis) growth. The power–time curves of the growth metabolism of B. subtilis affected by Cornu Cervi Pantotrichum, Cornu Cervi Elaphi, Cornu Saigae Tataricae, cornu caprae hircus, Cornu Bubali, Squama Manis, and Carapax Trionycis were determined using a thermal activity monitor (TAM) air isothermal microcalorimeter, ampoule mode, at 37 °C. By analyzing these curves and some quantitative parameters using principal component analysis, the effects of the seven animal drugs on B. subtilis could be quickly evaluated from the change of the two main parameters, the maximum heat-flow power P 2m and total heat output Q t: Cornu Saigae Tataricae, cornu caprae hircus, Cornu Bubali, Squama Manis, and Carapax Trionycis inhibited the growth of B. subtilis, while Cornu Cervi Pantotrichum and Cornu Cervi Elaphi promoted the growth of B. subtilis. Further, the result of hierarchical clustering analysis showed that the drugs which promoted the growth of B. subtilis gathered in one cluster, the other drugs which inhibited the growth of B. subtilis gathered in the other cluster. All these illustrated that the internal characteristics of the seven animal drugs were different though they had similar resources and these drugs could be well clustered according the effects of them on B. subtilis growth with the help of chemometric methods. This study provided an useful idea of the combination of microcalorimetry and chemometric analysis for studying the effects of drugs on organisms.

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Acknowledgements

We are grateful to the support of National Basic Research Program of China (973 project) (Nos. 2007CB512607 and 2006CB504703) and National Nature Science Foundation (No. 30873385).

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Authors and Affiliations

  1. China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing, 100039, People’s Republic of China

    Xiaoyan Xing, Yanling Zhao, Weijun Kong, Dan Yan, Ping Zhang, Yumei Han, Lei Jia, Cheng Jin & Xiaohe Xiao

  2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, Sichuan, People’s Republic of China

    Xiaoyan Xing, Weijun Kong, Yumei Han & Lei Jia

  3. Viral Hepatitis Research Laboratory, Institute of Infectious Diseases, Beijing 302 Hospital, Beijing, 100039, China

    Yanwei Zhong

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  1. Xiaoyan Xing
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  2. Yanling Zhao
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  10. Xiaohe Xiao
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Correspondence to Xiaohe Xiao.

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Xing, X., Zhao, Y., Kong, W. et al. Application of microcalorimetry and chemometric analysis. J Therm Anal Calorim 109, 381–385 (2012). https://doi.org/10.1007/s10973-011-1335-x

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  • DOI: https://doi.org/10.1007/s10973-011-1335-x

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