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Activity of ginsenoside Rh2 on the growth of mice splenic lymphocytes investigated by microcalorimetry and factor analysis

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Abstract

The power–time curves of mice splenic lymphocytes growth at 37 °C affected by ginsenoside Rh2 were determined by microcalorimetry using a 3114/3236 TAM air bioactivity monitor with ampoule mode. Then, the minimal inhibitory concentration (MIC) of Rh2 on splenic lymphocytes growth was determined by serial dilution method. From factor analysis (FA) on six quantitative thermokinetic parameters from the power–time curves, the activity of Rh2 on splenic lymphocytes could be quickly evaluated by analyzing the changes in the two main parameters: growth rate constant k, and maximum heat-output power, P m. The results showed that Rh2 had strong inhibitory activity on splenic lymphocytes growth, and this inhibitory activity was strengthened with increasing concentration of Rh2 in the concentration range of 1.0–32.0 μg mL−1. This strong inhibitory also could be confirmed from the MIC of 50.0 μg mL−1 of Rh2 on splenic lymphocytes growth in RPMI-1640 culture medium. This study illustrated that microcalorimetry could not only offer a useful method for evaluating the activity of drugs, but also serve as a quantitative, sensitive, and simple analytic tool for the evaluation of drugs on cell growth.

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Acknowledgements

This study was supported by the Foundation of National Basic Research Program of China (No. 2007CB512607) and the National Nature Science Foundation (No. 30772740).

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Authors and Affiliations

  1. Institute of Traditional Chinese Material Medica, People’s Liberation Army, 100, the 4th Ring Road, Beijing, 100039, People’s Republic of China

    Yanling Zhao, Jiabo Wang, Limei Shan, Dan Yan & Xiaohe Xiao

  2. Animal Laboratory Center, 302 Hospital of People’s Liberation Army, 100, the 4th Ring Road, Beijing, 100039, People’s Republic of China

    Ruisheng Li

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  1. Yanling Zhao
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  2. Jiabo Wang
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  3. Limei Shan
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  4. Ruisheng Li
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  5. Dan Yan
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Correspondence to Xiaohe Xiao.

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Zhao, Y., Wang, J., Shan, L. et al. Activity of ginsenoside Rh2 on the growth of mice splenic lymphocytes investigated by microcalorimetry and factor analysis. J Therm Anal Calorim 105, 1037–1041 (2011). https://doi.org/10.1007/s10973-010-1146-5

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  • DOI: https://doi.org/10.1007/s10973-010-1146-5

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