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A Synergistic Combination Against Chronic Myeloid Leukemia: An Intra-molecular Mechanism of Communication in BCR–ABL1 Resistance

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Abstract

The constitutive BCR–ABL1 active protein fusion has been identified as the main cause of chronic myeloid leukemia. The emergence of T334I and D381N point mutations in BCR–ABL1 confer drug resistance. Recent experimental studies show a synergistic effect in suppressing this resistance when Nilotinib and Asciminib are co-administered to target both the catalytic and allosteric binding site of BCR–ABL1 oncoprotein, respectively. However, the structural mechanism by which this synergistic effect occurs has not been clearly elucidated. To obtain insight into the observed synergistic effect, molecular dynamics simulations have been employed to investigate the inhibitory mechanism as well as the structural dynamics that characterize this effect. Structural dynamic analyses indicate that the synergistic binding effect results in a more compact and stable protein conformation. In addition, binding free energy calculation suggests a dominant energy effect of nilotinib during co-administration. van der Waals energy interactions were observed to be the main energy component driving this synergistic effect. Furthermore, per-residue energy decomposition analysis identified Glu481, Ser453, Ala452, Tyr454, Phe401, Asp400, Met337, Phe336, Ile334, And Val275 as key residues that contribute largely to the synergistic effect. The findings highlighted in this study provide a molecular understanding of the dynamics and mechanisms that mediate the synergistic inhibition in BCR–ABL1 protein in chronic myeloid leukemia treatment.

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Acknowledgements

The authors acknowledge the College of Health Science, the University of KwaZulu-Natal for financial support and Centre of High-Performance Computing (CHPC) Cape Town, RSA, for computational resources (http://www.chpc.ac.za).

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Authors and Affiliations

  1. Molecular Bio-computation and Drug Design Lab, School of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban, 4001, South Africa

    Ahmed A. El Rashedy, Patrick Appiah-Kubi & Mahmoud E. S. Soliman

  2. College of Pharmacy and Pharmaceutical Sciences, Florida Agricultural and Mechanical University, FAMU, Tallahassee, FL, 32307, USA

    Mahmoud E. S. Soliman

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  1. Ahmed A. El Rashedy
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Correspondence to Mahmoud E. S. Soliman.

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El Rashedy, A.A., Appiah-Kubi, P. & Soliman, M.E.S. A Synergistic Combination Against Chronic Myeloid Leukemia: An Intra-molecular Mechanism of Communication in BCR–ABL1 Resistance. Protein J 38, 142–150 (2019). https://doi.org/10.1007/s10930-019-09820-z

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