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Biocompatibility and Antimicrobial Investigation of Agar-Tannic Acid Hydrogel Reinforced with Silk Fibroin and Zinc Manganese Oxide Magnetic Microparticles

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Abstract

Herein, a three-dimensional cross-linked hydrogel was synthesized based on agarose (Agr) and tannic acid (TA) biopolymers which are cross-linked with Zn2+ ions. Also, the extracted silk fibroin (SF) and zinc manganese oxide (ZnMn2O4) magnetic microparticles were added to the composite to be utilized in biomedical applications. It was aimed to prepare a biocomposite based on the natural materials due to their various biological characteristics owing to their less toxicity and reduced hemolysis. Therefore, this novel biocomposite scaffold (Agr-TA/SF/ZnMn2O4) demonstrated desirable biological capacity characterized by cell viability approach, hemolysis assay, and anti-biofilm activity. Due to the cell viability test results, after 3 and 7 days, the viability of the Hu02 cell in the presence of Agr-TA/SF/ZnMn2O4 scaffold was 95.38% and 93.83%, respectively. Since the hemolytic activity below 5% is considered hemocompatible, the prepared biocomposite with 4.97% hemolytic activity is totally hemocompatible. Moreover, according to the considerable anti-biofilm activity, along with restraining the P. aeruginosa biofilm formation, this cross-linked hydrogel has been administrated as an excellent candidate for biological applications.

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The authors gratefully acknowledge the partial support from the Research Council of the Iran University of Science and Technology.

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Authors and Affiliations

  1. Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran

    Fatemeh Ganjali, Ali Maleki & Seyed Masoud Hashemi

  2. Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran

    Reza Eivazzadeh-Keihan & Reza Ahangari Cohan

  3. Protein Chemistry Laboratory, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

    Hooman Aghamirza Moghim Aliabadi & Saeedeh Pouri

  4. Advanced Chemical Studies Laboratory, Department of Chemistry, K. N. Toosi University of Technology, Tehran, Iran

    Hooman Aghamirza Moghim Aliabadi

  5. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Mahdavi

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  1. Fatemeh Ganjali
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Ganjali, F., Eivazzadeh-Keihan, R., Aghamirza Moghim Aliabadi, H. et al. Biocompatibility and Antimicrobial Investigation of Agar-Tannic Acid Hydrogel Reinforced with Silk Fibroin and Zinc Manganese Oxide Magnetic Microparticles. J Inorg Organomet Polym 32, 4057–4069 (2022). https://doi.org/10.1007/s10904-022-02410-0

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  • DOI: https://doi.org/10.1007/s10904-022-02410-0

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