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Supramolecular Linear Assemblies of Cytochrome b 562 Immobilized on a Gold Electrode

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Abstract

Linear assemblies of cytochrome b 562 (cyt b 562) containing a chemically-modified heme molecule on the protein surface were constructed by successive intermolecular heme–heme pocket interactions and then immobilized onto a gold electrode via a heme molecule anchored on the electrode. The accumulation of the cyt b 562 units were confirmed by electrochemical analyses. The average number of proteins in each assembly was estimated to be about 6 units by quartz crystal microbalance and atomic force microscopy (AFM) analyses. The linear assemblies of the cyt b 562 units were clearly visualized by AFM as a rod-like architecture with a vertical orientation to the electrode surface.

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References

  1. I. Willner, B. Willner, E. Katz, Bioelectrochemistry 70, 2 (2007)

    Article  CAS  Google Scholar 

  2. N. Terasaki, N. Yamamoto, T. Hiraga, Y. Yamanoi, T. Yonezawa, H. Nishihara, T. Ohmori, M. Sakai, M. Fujii, A. Tohri, M. Iwai, Y. Inoue, S. Yoneyama, M. Minakata, I. Enami, Angew. Chem. Int. Ed. 48, 1585 (2009)

    Article  CAS  Google Scholar 

  3. M. Miyachi, Y. Yamanoi, Y. Shibata, H. Matsumoto, K. Nakazato, M. Konno, K. Ito, Y. Inoue, H. Nishihara, Chem. Commun. 46, 2557 (2010)

    Article  CAS  Google Scholar 

  4. C.F. Meunier, X.Y. Yang, J.C. Rooke, B.L. Su, Chem. Cat. Chem 3, 476 (2011)

    CAS  Google Scholar 

  5. T. Nöll, G. Nöll, Chem. Soc. Rev. 40, 3564 (2011)

    Article  Google Scholar 

  6. C. Léger, P. Bertrand, Chem. Rev. 108, 2379 (2008)

    Article  Google Scholar 

  7. A.J. Gates, G.L. Kemp, C.Y. To, J. Mann, S.J. Marritt, A.G. Mayes, D.J. Richardson, J.N. Butt, Phys. Chem. Chem. Phys. 13, 7720 (2011)

    Article  CAS  Google Scholar 

  8. K. Nakano, T. Yoshitake, Y. Yamashita, E.F. Bowden, Langmuir 23, 6270 (2007)

    Article  CAS  Google Scholar 

  9. Y. Tokita, S. Yamada, W. Luo, Y. Goto, N. Bouley-Ford, H. Nakajima, Y. Watanabe, Angew. Chem. Int. Ed. 50, 11663 (2011)

    Article  CAS  Google Scholar 

  10. Y. Tokita, J. Shimura, H. Nakajima, Y. Goto, Y. Watanabe, J. Am. Chem. Soc. 130, 5302 (2008)

    Article  CAS  Google Scholar 

  11. N. Terasaki, M. Iwai, N. Yamamoto, T. Hiraga, S. Yamada, Y. Inoue, Thin Solid Films 516, 2553 (2008)

    Article  CAS  Google Scholar 

  12. C. Ley, D. Holtmann, K.M. Mangold, J. Schrader, Colloids Surf. B 88, 539 (2011)

    Article  CAS  Google Scholar 

  13. M. Zayats, E. Katz, R. Baron, I. Willner, J. Am. Chem. Soc. 127, 12400 (2005)

    Article  CAS  Google Scholar 

  14. Y. Xiao, F. Patolsky, E. Katz, J.F. Hainfeld, I. Willner, Science 299, 1877 (2003)

    Article  CAS  Google Scholar 

  15. A. Liu, M. Wei, I. Honma, H. Zhou, Anal. Chem. 77, 8068 (2005)

    Article  CAS  Google Scholar 

  16. A. Jing, Y. Tan, Y. Wang, S. Ding, W. Qian, J. Nanosci. Nanotechnol. 7, 440 (2007)

    Article  CAS  Google Scholar 

  17. R. Dronov, D.G. Kurth, H. Möhwald, R. Spricigo, S. Leimkühler, U. Wollenberger, K.V. Rajagopalan, F.W. Scheller, F. Lisdat, J. Am. Chem. Soc. 130, 1122 (2008)

    Article  CAS  Google Scholar 

  18. P.N. Ciesielski, C.J. Faulkner, M.T. Irwin, J.M. Gregory, N.H. Tolk, D.E. Cliffel, G.K. Jennings, Adv. Funct. Mater. 20, 4048 (2010)

    Article  CAS  Google Scholar 

  19. F. Lisdat, R. Dronov, H. Möhwald, F.W. Scheller, D.G. Kurth, Chem. Commun. 274 (2009)

  20. L. Frolov, O. Wilner, C. Carmeli, I. Carmeli, Adv. Mater. 20, 263 (2008)

    Article  CAS  Google Scholar 

  21. P.N. Ciesielski, A.M. Scott, C.J. Faulkner, B.J. Berron, D.E. Cliffel, G.K. Jennings, ACS Nano 2, 2465 (2008)

    Article  CAS  Google Scholar 

  22. O.A. Raitman, F. Patolsky, E. Katz, I. Willner, Chem. Commun. 1936 (2002)

  23. R. Dronov, D.G. Kurth, H. Möhwald, F.W. Scheller, F. Lisdat, Angew. Chem. Int. Ed. 47, 3000 (2008)

    Article  CAS  Google Scholar 

  24. D.H. Murgida, P. Hildebrandt, Phys. Chem. Chem. Phys. 7, 3773 (2005)

    Article  CAS  Google Scholar 

  25. K. Kobayashi, M. Shimizu, T. Nagamune, H. Sasabe, Y. Fang, W. Knoll, Bull. Chem. Soc. Jpn. 75, 1707 (2002)

    Article  CAS  Google Scholar 

  26. J. Xu, F. Shang, J.H.T. Luong, K.M. Razeeb, J.D. Glennon, Biosens. Bioelectron. 25, 1313 (2010)

    Article  CAS  Google Scholar 

  27. I. Taniguchi, K. Watanabe, M. Tominaga, J. Electroanal. Chem. 333, 331 (1992)

    Article  CAS  Google Scholar 

  28. A.E. Nassar, W.S. Willis, J.F. Rusling, Anal. Chem. 67, 2386 (1995)

    Article  CAS  Google Scholar 

  29. F.W. Scheller, U. Wollenberger, C. Lei, W. Jin, B. Ge, C. Lehmann, F. Lisdat, V. Fridman, Rev. Mol. Biotech. 82, 411 (2002)

    Article  CAS  Google Scholar 

  30. E.E. Ferapontova, Electroanalysis 16, 1101 (2004)

    Article  CAS  Google Scholar 

  31. Y. Wu, S. Hu, Microchim. Acta 159, 1 (2007)

    Article  CAS  Google Scholar 

  32. N. Bistolas, U. Wollenberger, C. Jung, F.W. Scheller, Biosens. Bioelectron. 20, 2408 (2005)

    Article  CAS  Google Scholar 

  33. G.X. Ma, T.H. Lu, Y.Y. Xia, Bioelectrochemistry 71, 180 (2007)

    Article  CAS  Google Scholar 

  34. A. Ramanavicius, A. Ramanaviciene, Fuel Cells 9, 25 (2009)

    Article  CAS  Google Scholar 

  35. A. Onoda, Y. Kakikura, T. Uematsu, S. Kuwabata, T. Hayashi, Angew. Chem. Int. Ed. 51, 2628 (2012)

    Article  CAS  Google Scholar 

  36. A. Onoda, Y. Ueya, T. Sakamoto, T. Uematsu, T. Hayashi, Chem. Commun. 46, 8 (2010)

    Article  Google Scholar 

  37. A. Onoda, T. Himiyama, K. Ohkubo, S. Fukuzumi, T. Hayashi, Chem. Commun. 48, 8054 (2012)

    Article  CAS  Google Scholar 

  38. The “heme” used in this paper includes both Fe(II) and Fe(III) complexes of protoporphyrin IX

  39. L. Fruk, C.H. Kuo, E. Torres, C.M. Niemeyer, Angew. Chem. Int. Ed. 48, 1550 (2009)

    Article  CAS  Google Scholar 

  40. H. Kitagishi, K. Oohora, H. Yamaguchi, H. Sato, T. Matsuo, A. Harada, T. Hayashi, J. Am. Chem. Soc. 129, 10326 (2007)

    Article  CAS  Google Scholar 

  41. H. Kitagishi, K. Oohora, T. Hayashi, Biopolymers 91, 194 (2009)

    Article  CAS  Google Scholar 

  42. H. Kitagishi, Y. Kakikura, H. Yamaguchi, K. Oohora, A. Harada, T. Hayashi, Angew. Chem. Int. Ed. 48, 1271 (2009)

    Article  CAS  Google Scholar 

  43. K. Oohora, A. Onoda, H. Kitagishi, H. Yamaguchi, A. Harada, T. Hayashi, Chem. Sci. 2, 1033 (2011)

    Article  CAS  Google Scholar 

  44. F.W.J. Teale, Biochim. Biophys. Acta 35, 543 (1959)

    Article  CAS  Google Scholar 

  45. S. Takeda, N. Kamiya, T. Nagamune, Biotechnol. Lett. 26, 121 (2004)

    Article  CAS  Google Scholar 

  46. H. Zimmermann, A. Lindgren, W. Schuhmann, L. Gorton, Chem. Eur. J. 6, 592 (2000)

    Article  CAS  Google Scholar 

  47. A. Das, S.A. Trammell, M.H. Hecht, Biophys. Chem. 123, 102 (2006)

    Article  CAS  Google Scholar 

  48. G.R. Moore, R.J.P. Williams, J. Peterson, A.J. Thomson, F.S. Mathews, Biochim. Biophys. Acta 829, 83 (1985)

    Article  CAS  Google Scholar 

  49. E. Itagaki, L.P. Hager, J. Biolog, Chem. 241, 3687 (1966)

    CAS  Google Scholar 

  50. Q. Wang, F. Zhi, W. Wang, X. Xia, X. Liu, F. Meng, Y. Song, C. Yang, X. Lu, J. Phys. Chem. C 113, 9359 (2009)

    Article  CAS  Google Scholar 

  51. A. Onoda, H. Nagai, S. Koga, T. Hayashi, Bull. Chem. Soc. Jpn. 83, 375 (2010)

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research ((B) and Innovative Areas “Coordination Programming”, area 2107) from MEXT and the Japan Society for the Promotion of Science (JSPS). Y.K. acknowledges a support from the Global COE Program of Osaka University and from JSPS. A.O. acknowledges a support from the Frontier Research Base for Global Young Researchers, Osaka University, on the Program of MEXT, and from the Ogasawara Foundation. T.H. acknowledges a support from the Asahi Grass Foundation.

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Author notes

  1. Hiroaki Kitagishi

    Present address: Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto, 610-0321, Japan

Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Yasuaki Kakikura, Akira Onoda, Emi Kubo, Hiroaki Kitagishi, Taro Uematsu, Susumu Kuwabata & Takashi Hayashi

  2. Frontier Research Base for Global Young Researchers, Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan

    Akira Onoda

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  1. Yasuaki Kakikura
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  2. Akira Onoda
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Correspondence to Akira Onoda or Takashi Hayashi.

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This paper is dedicated to Prof. Dr. Hiroshi Nishihara for his outstanding contribution to the field of metal-containing polymers.

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Kakikura, Y., Onoda, A., Kubo, E. et al. Supramolecular Linear Assemblies of Cytochrome b 562 Immobilized on a Gold Electrode. J Inorg Organomet Polym 23, 172–179 (2013). https://doi.org/10.1007/s10904-012-9737-1

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  • DOI: https://doi.org/10.1007/s10904-012-9737-1

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