Abstract
Fluorescent tools have revolutionized our capability to visualize, probe, study, and understand the biological cellular properties, processes and dynamics, enabling researchers to improve their knowledge for example in cancer field. In this paper, we use the peculiar properties of our Imiqualines derivatives to study their cellular penetration and distribution in a human melanoma cell line A375 using confocal microscopy. Preliminary results on colocalization with the potent protein target c-Kit of our lead are also described.
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Acknowledgements
We would like to thank Marjorie Damian from the team Cellular Pharmacology (IBMM, Montpellier, France) directed by Dr. Jean-Louis Banères for technical support to record UV and fluorescence spectra. We also thank Marie-Pierre Blanchard, head of the MRI Imaging Platform at the Functional Genomics Institute belonging to the Biocampus of Montpellier (France), for having recorded all the data relating to confocal microscopy.
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Patinote, C., Cirnat, N., Hadj-Kaddour, K. et al. Substantial Cellular Penetration of Fluorescent Imidazoquinoxalines. J Fluoresc 30, 1499–1512 (2020). https://doi.org/10.1007/s10895-020-02595-y
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DOI: https://doi.org/10.1007/s10895-020-02595-y