Abstract
The first observation of Metal-Enhanced Fluorescence (MEF) from large gold colloids is presented. Gold colloids, 40 and 200 nm diameter, were deposited onto glass substrates in a homogeneous fashion. The angular-dependent fluorescence emission of FITC-HSA, adsorbed onto gold colloids, was measured on a rotating stage which was used to evaluate MEF at all spatial angles. The emission intensity of FITC-HSA was found to be up to 2.5-fold brighter than the emission on bare glass substrates at an angle of 270 degrees. This is explained by the Radiating Plasmon Model, whereby the combined system, composed of the fluorophore and the metal colloids, emits with the photophysical characteristics of the fluorophore, after the excitation and the partial radiationless energy transfer between the excited states of the fluorophore and the surface plasmons of the gold colloids. The fluorescence enhancement was found to be higher with 200 nm gold colloids as compared to 40 nm colloids due to the increased contribution of the scattering portion of the 200 nm gold colloid extinction spectrum. These observations suggest that gold colloids could be used in MEF applications, offering more stable surfaces than the commonly used silvered surfaces, for applications requiring longer term storage and use.
Abbreviations
- HSA:
-
Human serum albumin
- MEF:
-
Metal-Enhanced Fluorescence
- RPM:
-
Radiating Plasmon Model
References
Aslan K, Gryczynski I, Malicka J, Lakowicz JR, Geddes CD (2005) Metal-enhanced fluorescence: An emerging tool in biotechnology. Curr Opin Biotechnol 16(1):55–62
Aslan K, Gryczynski I, Malicka J, Lakowicz JR, Geddes CD (2005) In: Shayne G (ed) Drug discovery handbook. Wiley, New Jersey, pp 603–666
Aslan K, Holley P, Geddes CD (2006) Microwave-accelerated metal enhanced fluorescence (MAMEF) with silver colloids in 96-well plates: Application to ultra fast and sensitive immunoassays, High Throughput Screening and drug discovery. J Immunol Methods 312:137–147
Geddes CD, Aslan K, Gryczynski I, Malicka J, Lakowicz JR (2004) In: Geddes CD, Lakowicz JR (eds) Reviews in fluorescence 2004. Kluwer Academic/Plenum Publishers, New York, pp 365–401
Aslan K, Geddes CD (2006) Microwave accelerated and metal enhanced fluorescence myoglobin detection on silvered surfaces: Potential application to myocardial infarction diagnosis. Plasmonics 1(1):53–59
Geddes CD, Aslan K, Gryczynski I, Malicka J, Lakowicz JR (2005) Radiative decay engineering. In: Geddes CD, Lakowicz JR (eds) Topics in fluorescence spectroscopy. Kluwer Academic/Plenum Publishers, New York, pp 401–448
Malicka J, Gryczynski I, Geddes CD, Lakowicz JR (2003) Metal-enhanced emission from indocyanine green: A new approach to in vivo imaging. J Biomed Opt 8(3):472–478
Geddes CD, Cao H, Gryczynski I, Grcyzynski Z, Fang J, Lakowicz JR (2003) Metal-enhanced fluorescence (MEF) due to silver colloids on a planar surface: Potential applications of indocyanine green to in vivo imaging. J Phys Chem A 107(18):3443–3449
Aslan K, Leonenko Z, Lakowicz JR, Geddes CD (2005) Fast and slow deposition of silver nanorods on planar surfaces: Application to metal-enhanced fluorescence. J Phys Chem B 107(13):6247–6251
Aslan K, Leonenko Z, Lakowicz JR, Geddes CD (2005) Rapid deposition of triangular silver nanoplates on planar surfaces: Application to metal-enhanced fluorescence. J Phys Chem B 109(8):3157–3162
Parfenov A, Gryczynski I, Malicka J, Geddes CD, Lakowicz JR (2003) Enhanced fluorescence from fluorophores on fractal silver surfaces. J Phys Chem B 107:8829–8833
Parfenov A, Geddes CD, Lakowicz JR (2003) Photodeposition of silver can result in metal-enhanced fluorescence. Appl Spectrosc 57(5):526–531
Geddes CD, Parfenov A, Roll D, Fang J, Lakowicz JR (2003) Electrochemical and laser deposition of silver for use in metal-enhanced fluorescence. Langmuir 19(15):6236–6241
Aslan K, Badugu R, Geddes CD (2005) Metal-enhanced fluorescence from plastic substrates. J Fluoresc 15(2):99–104
Geddes CD, Parfenov A, Roll D, Gryczynski I, Malicka J, Lakowicz JR (2004) Spectrochimica Acta Part A 60(8–9):1977–1983
Aslan K, Leonenko Z, Lakowicz JR, Geddes CD (2005) Annealed silver-island films for applications in metal-enhanced fluorescence: Interpretation in terms of radiating plasmons. J Fluoresc 15(5):643–654
Aslan K, Perez-Luna VH (2004) Quenched emission of fluorescence by ligand functionalized gold nanoparticles. J Fluoresc 14:401–405
Dulkeith E, Morteani AC, Niedereichholz T, Klar TA, Feldmann J, Levi SA, van Veggel FCJM, Reinhoudt DN, Moller M, Gittins DI (2002) Fluorescence quenching of dye molecules near gold nanoparticles: Radiative and nonradiative effects. Phys Rev Lett 89:203002
Dulkeith E, Ringler M, Klar TA, Feldmann J, Javier AM, Parak WJ (2005) Gold nanoparticles quench fluorescence by phase induced radiative rate suppression. Nano Lett 5(4):585–589
Yguerabide J, Yguerabide E (1998) Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications—I. Theor Anal Biochem 262:137–156
Yguerabide J, Yguerabide E (1998) Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications—II. Experimental characterization. Anal Biochem 262:157–176
Aslan K, Holley P, Davies L, Lakowicz JR, Geddes CD (2005) Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensing. J Am Chem Soc 127:12115–12121
Mie G (1908) Ann Phys 25:377–445
Lakowicz JR (2001) Radiative decay engineering: biophysical and biomedical applications. Anal Biochem 298;1–24
Lakowicz JR (2005) Radiative decay engineering 5: Metal-enhanced fluorescence and plasmon emission. Anal Biochem 337:171–194
Liebermann T, Knoll W (2000) Surface-plasmon field-enhanced fluorescence spectroscopy colloids and surfaces. A: Physicochemical and Eng Aspects 171:115–130
Lakowicz JR (2004) Radiative decay engineering 3. Surface plasmon-coupled directional emission. Anal Biochem 324:153–169
Lakowicz JR (1999) Principles of fluorescence spectroscopy. Kluwer, New York
Acknowledgments
KA and CDG acknowledges UMBI, the CFS (to CDG) and the IoF for salary support. Additional support from the National Center for Research Resources, RR- 08119 is also acknowledged.
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Aslan, K., Malyn, S.N. & Geddes, C.D. Metal-Enhanced Fluorescence from Gold Surfaces: Angular Dependent Emission. J Fluoresc 17, 7–13 (2007). https://doi.org/10.1007/s10895-006-0149-x
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DOI: https://doi.org/10.1007/s10895-006-0149-x