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L-Glutamate in formation of long-term memory in the honeybee Apis mellifera

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Abstract

Participation of metabotropic glutamate receptors (mGluR) in associative learning of the honeybee Apis mellifera was studied in behavioral and electrophysiological experiments with use of pharmacological analysis. By the method of conditioned reflexes, effects of systemic injections of mGluR agonists and antagonists were studied on retention of elaborated conditioned reflexes in short-term and long-term memory. Injection of aminocyclopentandicarbonic acid (ACPD), ibotenate, and phosphoserine stimulated the memory in 3 h after the single learning procedure. The long- term memory was inhibited by mGluR antagonists — methylcarboxyphenylglycine and amino- phosphonopropionate as well as by non-competitive antagonists of mGluR1(CPCCOEt) and of mGluR5 (SIB 1757). Electrophysiological experiments demonstrated changes of characteristics of the action potential recorded from mushroom bodxies in response to stimulation of antennal lobes and during injections of mGluR III phosphoserine and the mGluR1 antagonist CPCCOEt. The obtained data allow suggesting participation of different groups of metabotropic glutamate receptors in the process of the honeybee associative learning.

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    N. G. Lopatina, I. V. Ryzhova, T. G. Zachepilo, V. B. Smirnov & E. G. Chesnokova

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  1. N. G. Lopatina
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  2. I. V. Ryzhova
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  3. T. G. Zachepilo
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  4. V. B. Smirnov
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  5. E. G. Chesnokova
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Translated from Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, Vol. 40, No. 6, 2004, pp. 539–545.

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Lopatina, N.G., Ryzhova, I.V., Zachepilo, T.G. et al. L-Glutamate in formation of long-term memory in the honeybee Apis mellifera . J Evol Biochem Phys 40, 662–670 (2004). https://doi.org/10.1007/s10893-004-0007-x

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  • DOI: https://doi.org/10.1007/s10893-004-0007-x

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