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Bisorbicillinoids Produced by the Fungus Trichoderma citrinoviride Affect Feeding Preference of the Aphid Schizaphis graminum

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Abstract

We report the effects of some bisorbicillinoids isolated from biomass of the fungus Trichoderma citrinoviride on settling and feeding preference of the aphid Schizaphis graminum. Purification of the fungal metabolites was carried out by a combination of column chromatography and thin-layer chromatography using direct and reverse phases. Chemical identification was performed by spectroscopic methods including nuclear magnetic resonance and mass spectrometry. The identified bisorbicillinoids appeared to be bislongiquinolide, its 16,17-dihydro derivative, trichodimerol, and dihydrotrichodimerol. A feeding preference test with alate morphs of S. graminum was used to identify the active fractions. Among the four bisorbicillinoids, dihydrotrichodimerol and bislongiquinolide influenced aphid feeding preference, restraining specimens from settling on leaves treated with metabolites. Taste neurons sensitive to these compounds, particularly to bislongiquinolide, were located on tarsi of the S. graminum alate morphs.

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Acknowledgment

The NMR spectra were recorded at the NMR Service of the Istituto di Chimica Biomolecolare del CNR, Pozzuoli, Italy. Dr. G. Ricciardiello and Mrs D. Melck are gratefully acknowledged for technical assistance and Mrs. M. Zampa for recording MS spectra. The authors also acknowledge the assistance of Dr. Pasqualina Grazioso for statistical analysis. The work was supported by a grant from the Italian Ministry of University and Research (MIUR). Contribution DISSPAPA n. 162.

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Authors and Affiliations

  1. Dipartimento di Scienze del Suolo, della Pianta, dell’Ambiente e delle Produzioni Animali, Università di Napoli Federico II, Via Università 100, 80055, Portici, Italy

    Antonio Evidente, Anna Andolfi & Alessio Cimmino

  2. Dipartimento di Biologia Animale, Università di Modena e Reggio Emilia, Via Campi 213/D, 41100, Modena, Italy

    Sonia Ganassi & Maria Agnese Sabatini

  3. Istituto di Scienze delle Produzioni Alimentari del CNR, Via Amendola, 122/O, 70126, Bari, Italy

    Claudio Altomare & Mara Favilla

  4. Dipartimento di Scienze Animali, Vegetali e dell’Ambiente, Università del Molise, Via De Sanctis, 86100, Campobasso, Italy

    Antonio De Cristofaro & Silvia Vitagliano

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  1. Antonio Evidente
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  9. Maria Agnese Sabatini
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Correspondence to Antonio Evidente.

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Evidente, A., Andolfi, A., Cimmino, A. et al. Bisorbicillinoids Produced by the Fungus Trichoderma citrinoviride Affect Feeding Preference of the Aphid Schizaphis graminum . J Chem Ecol 35, 533–541 (2009). https://doi.org/10.1007/s10886-009-9632-6

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  • DOI: https://doi.org/10.1007/s10886-009-9632-6

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