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Development and Characterization of Phytoniosome Nano Vesicle Loaded with Aqueous Leaf Extracts of Justicia adhatoda and Psidium guajoava Against Dengue Virus (DEN-2)

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Abstract

The leaves of Psidium guajava (PG) and Justicia adhatoda (JA) are traditionally used in the Indian herbal medicine for the treatment of dengue. The current research work was aimed to evaluate the antiviral activity of the phytoniosomes (PN) loaded with the aqueous extracts of PG and JA (AGN) against dengue virus-2 strain (DEN-2). The AGN were prepared by ultrasonication method using tween 80, poloxamer 407, polyethylene glycol 6000 and cholesterol. The particle size of the PN was found out to be in the range of 105.1 ± 3.6–279.4 ± 5.2 nm. In comparison to the non-PEGylated niosomes, PEGylated niosomes showed lower particle size. AGN showed in vitro antiviral activity against vero cells infected with DEN-2 virus. Capsules containing AGN powder were found to be stable for 3 months when stored at room temperature. AGN4 showed maximum of 40% plaque forming units, indicating that formulation poses significant viral inhibition in in vitro condition.

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Authors and Affiliations

  1. Department of Pharmaceutics, PSG College of Pharmacy, Coimbatore, Tamil Nadu, 641 004, India

    Dhanya K. Wilson, Karthik Siram, Arjunan Karuppaiah & Veintramuthu Sankar

  2. Department of Pharmacognosy, PSG College of Pharmacy, Coimbatore, Tamil Nadu, 641 004, India

    Govindarajan Shyamala

  3. Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India

    Manickam Paulpandi & Arul Narayanasamy

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  1. Dhanya K. Wilson
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Wilson, D.K., Shyamala, G., Paulpandi, M. et al. Development and Characterization of Phytoniosome Nano Vesicle Loaded with Aqueous Leaf Extracts of Justicia adhatoda and Psidium guajoava Against Dengue Virus (DEN-2). J Clust Sci 32, 297–304 (2021). https://doi.org/10.1007/s10876-020-01788-6

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  • DOI: https://doi.org/10.1007/s10876-020-01788-6

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