Abstract
This study highlights the apoptosis-inducing ability of eco-friendly phyto-synthesis route for silver nanoparticles (AgNPs) by using Artemisia tschernieviana extract (ATE). The aerial parts extract of A. tschernieviana was analysed for chemical composition using the gas chromatography–mass spectrometer (GC–MS) analysis. The phyto-synthesized AgNPs using A. tschernieviana extract (AgNP-ATE) were characterized by ultraviolet–visible, zeta potential, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The AgNP-ATE thus prepared was observed to have characteristic surface plasmon resonance peak at around 425 nm. The surface morphology and size of AgNPs show that the particles were mostly spherical and their sizes varied from 5 to 50 nm. The prepared AgNP-ATE showed more potent in vitro cytotoxicity against HT29 human colon adenocarcinoma cell line than normal human embryonic kidney (HEK293) cells. Moreover, studies such as Fluorescein isothiocyanate-annexin V and propidium iodide staining, caspase assay, and Hoecht 33258 staining demonstrate that the prepared AgNP-ATE shows significantly enhanced apoptosis in human colon cancer cells as compared to untreated cells. The current data suggest that the phyto-synthesized AgNPs show effective anticancer properties against HT29 cells.
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Khalili, H., Sadat Shandiz, S.A. & Baghbani-Arani, F. Anticancer Properties of Phyto-Synthesized Silver Nanoparticles from Medicinal Plant Artemisia tschernieviana Besser Aerial Parts Extract Toward HT29 Human Colon Adenocarcinoma Cells. J Clust Sci 28, 1617–1636 (2017). https://doi.org/10.1007/s10876-017-1172-6
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DOI: https://doi.org/10.1007/s10876-017-1172-6