Abstract
The impact of gamma irradiation on growth and physiology of Euryale ferox was described in the present investigation. E. ferox is an underutilized aquatic food crop that grows in shallow-water bodies in lower Assam regions and north Bihar of India. The seeds of E. ferox were irradiated with different doses of gamma irradiation ranging from 0 to 500 Gy. It was observed that the germination and survival percentage was inhibited by increasing the irradiation dose. However, plants developed from seed exposed to an irradiation dose beyond 100 Gy did not survive more than 1 month. Further growth parameters (leaf size and number, number of thorns, root number and length, and number of flower and seeds) were also compared with respect to non-irradiated plants. Physiological parameters, viz. chlorophyll a, chlorophyll b, total chlorophyll, photosynthetic rate, transpiration rate, stomatal conductance, and intracellular CO2 content was higher in the irradiation population of E. ferox. Superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities were observed low in irradiated population of E. ferox. The proline and glycine betaine content was enhanced with increasing the irradiation dose. The present investigation explores the potential use of gamma rays in genetic improvement of E. ferox and improves understanding of the physiological responses inflicted by gamma irradiation.
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References
Boyd, C.E.: Fresh water plants: a potential source of protein. Econ. Bot. 22, 359–368 (1968)
Mandal, R.N., Saha, G.S., Sarangi, N.: Harvest and processing of Makhana (Euryale ferox Salisb.)—a unique assemblage of traditional knowledge. Indian J. Tradit. Knowl. 9, 684–688 (2010)
Nath, B.K., Chakraborty, A.K.: Studies on amino acid composition of the seeds of Euryale ferox Salisb. J. Food Sci. Technol. 22, 293 (1985)
Jha, V., Barat, G.K., Jha, U.N.: Nutritional evalution of Euryale ferox Salisb. (Makhana). J. Food Sci. Technol. 28(5), 326–328 (1991)
Jha, V., Barat, G.K.: Nutritional and medicinal properties of Euryale ferox Salisbury. In: Mishra, R.K., Jha, V., Dehadrai, P.V. (eds.) Makhana, pp. 230–238. ICAR, New Delhi (2003)
Imanishi, A., Kaneko, S., Isagi, Y., Imanishi, J., Natuhara, Y., Morimoto, Y.: Development of microsatellite markers for Euryale ferox (Nymphaeaceae), an endangered aquatic plant species in Japan. Am. J. Bot. 98, 233–235 (2011)
Kumar, L., Gupta, V.K., Jha, B.K., Singh, I.S., Bhatt, B.P., Singh, A.K: Status of Makhana (Euryale ferox Salisb.) cultivation in India. Technical Bulletin No. R-32/PAT- 21, ICAR Research Complex for Eastern Region, Patna, India, pp. 31 (2011)
Verma, A.K., Banerji, B.K., Chakrabarty, D., Datta, S.K.: Studies on Makhana (Euryale ferox Salisbury). Curr. Sci. 99(6), 795–800 (2010)
Kovacs, E., Keresztes, A.: Effect of gamma and UV-B/C radiation on plant cell. Micron 33, 199–210 (2002)
Kumar, G., Gupta, P.: Mutagenic efficiency of lower doses of gamma rays in black cumin (Nigella sativa L.). Cytologia 72, 435–440 (2007)
Jan, S., Parween, T., Siddiqi, T.O.: Effect of gamma radiation on morphological, biochemical, and physiological aspects of plants and plant products. Environ. Reviews 20, 17–39 (2011)
Bhosale, R.S., More, A.D.: Effect of gamma radiation on seed germination, seedling height and seedling injury in Withania somnifera, (L.) Dunal. Int. J. Life Sci. 2, 226–228 (2014)
Verma, A.K., Sharma, S., Kakani, R.K., Meena, R.D., Choudhary, S.: Gamma radiation effects seed germination, plant growth and yield attributing characters of fennel (Foeniculum vulgare mill.). Int. J. Curr. Microbiol. App. Sci. 6(5), 2448–2458 (2017)
Hrscox, J.D., Israelstam, G.F.: A method for the extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot. 57, 1332–1334 (1979)
Aron, D.: Copper enzymes isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Beauchamp, C.O., Fridovich, I.: Superoxide dismutase; improved assays and an assay applicable to acrylamide gels. Ann. Biochem. 44, 276–287 (1971)
Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22, 867–880 (1981)
Bates, L.R., Waldren, R.P., Teare, I.D.: A rapid determination of free proline for water stress studies. Plant Soil 39, 205–207 (1973)
Grieve, C.M., Grattan, S.R.: Rapid assay for the determination of water soluble quaternary ammonium compounds. Plant Soil 70, 303–307 (1983). https://doi.org/10.1007/BF02374789
Somosy, Z.: Radiation response of cell organelles. Micron 31, 165–181 (2000)
Majeed, A., Khan, A.U.R., Ahmad, H., Muhammad, Z.: Gamma irradiation effect on some growth parameters of Lepidium sativum L. ARPN. J. Agric. Biol. Sci. 5(1), 39–42 (2010)
Ali, H., Ghori, Z., Sheikh, S., Gul, A.: Effects of Gamma Radiation on Crop Production. K.R. Hakeem (ed.), Crop Production and Global Environmental Issues, 27–78 (2015)
Marcu, D., Damian, G., Cosma, C., Cristea, V.: Gamma radiation effects on seed germination, growth and pigment content, and ESR study of induced free radicals in maize (Zea mays). J. Biol. Phys. 39, 625–634 (2013)
Chandrashekar, K.R., Somashekarappa, H.M., Souframanien, J.: Effect of gamma irradiation on germination, growth, and biochemical parameters of Terminalia arjuna Roxb. Radiat. Prot. Environ. 36, 38–44 (2017)
Celik, O., Atak, C., Suludere, Z.: Response of soybean plants to gamma radiation: biochemical analyses and expression patterns of trichome development. Plant Omics 7(5), 382–391 (2014)
Singh, B., Datta, P.S.: Effect of low dose gamma irradiation on plant and grain nutrition of wheat. Radiat. Phys. Chem. 79(8), 819–825 (2010)
McCord, J.M., Fridovich, I.: Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244(22), 6049–6055 (1969)
Kuznetsov, V.L., Shevyakova, N.I.: Polyamines and stress tolerance of plants. Plant Stress. 1, 50–71 (2007)
Cuin, A., Miller, A.J., Laurie, S.A., et al.: Potassium activities in cell compartments of salt-grown barley leaves. J. Exp. Bot. 54(383), 657–661 (2003)
Kumar, N., Pamidimarri, S.D.V.N., Kaur, M., Boricha, G., Reddy, M.P.: Effects of NaCl on growth, ion accumulation, protein, proline contents and antioxidant enzymes activity in callus cultures of Jatropha curcas. Biologia 63, 378–382 (2008)
Acknowledgements
The authors are thankful to Dr. Gautam Kumar for compilation of the project entitled “Genetic improvement of Makhana (Euryale ferox Salisbury) through gamma radiation”. This work is supported by a financial grant (Grant # 35/14/14/2014-BRNS) from BRNS-DAE, government of India, Mumbai, India.
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Kumar, N., Rani, S., Kuamr, G. et al. Physiological and biochemical responses of Makhana (Euryale ferox) to gamma irradiation. J Biol Phys 45, 1–12 (2019). https://doi.org/10.1007/s10867-018-9511-x
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DOI: https://doi.org/10.1007/s10867-018-9511-x