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pH effect on cellular uptake of Sn(IV) chlorine e6 dichloride trisodium salt by cancer cells in vitro

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Abstract

The effects of pH value and presence of serum in an incubation medium on photosensitizer drug cellular uptake in MCF7 cancer cells have been investigated. The results showed that the presence of serum in an incubation medium reduced the drug cellular uptake at all pH values. It has been found that decreasing on pH values of the incubation medium increased the cellular uptake of the drug, demonstrating selective uptake of the sensitizer. The HepG2 liver cancer cells exhibited more drug cellular uptake than CCD-18CO normal colon cells, which assessed the selectivity uptake of photosensitizer on cancerous cells. The concentration of photosensitizer measured in 106 cells showed a good correlation to the incubation time. Fluorescence and absorption spectroscopy been have used to examine the cells.

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Acknowledgements

The authors would like to thank Universiti Sains Malaysia for their supporting of this research work under grant No. 304/PFIZIK/638115, and to express their appreciation for the cooperation with the School of Biological Science, Universiti Sains Malaysia.

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Authors and Affiliations

  1. School of Physics, Universiti Sains Malaysia, 11800, Penang, Malaysia

    Khaled A. Al-Khaza’leh, Khalid Omar & M. S. Jaafar

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  1. Khaled A. Al-Khaza’leh
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  2. Khalid Omar
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  3. M. S. Jaafar
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Correspondence to Khalid Omar.

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Al-Khaza’leh, K.A., Omar, K. & Jaafar, M.S. pH effect on cellular uptake of Sn(IV) chlorine e6 dichloride trisodium salt by cancer cells in vitro. J Biol Phys 37, 153–161 (2011). https://doi.org/10.1007/s10867-010-9206-4

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  • DOI: https://doi.org/10.1007/s10867-010-9206-4

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