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The ectopic FOF1 ATP synthase of rat liver is modulated in acute cholestasis by the inhibitor protein IF1

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Abstract

Rat liver plasma membranes contain FOF1 complexes (ecto-FOF1) displaying a similar molecular weight to the mitochondrial FOF1 ATP synthase, as evidenced by Blue Native PAGE. Their ATPase activity was stably reduced in short-term extra-hepatic cholestasis. Immunoblotting and immunoprecipitation analyses demonstrated that the reduction in activity was not due to a decreased expression of ecto-FOF1 complexes, but to an increased level of an inhibitory protein, ecto-IF1, bound to ecto-FOF1. Since cholestasis down regulates the hepatic uptake of HDL-cholesterol, and ecto-FOF1 has been shown to mediate SR-BI-independent hepatic uptake of HDL-cholesterol, these findings provide support to the hypothesis that ecto-FOF1 contributes to the fine control of reverse cholesterol transport, in parallel with SR-BI. No activity change of the mitochondrial FOF1 ATP synthase (m-FOF1), or any variation of its association with m-IF1 was observed in cholestasis, indicating that ecto-IF1 expression level is modulated independently from that of ecto-FOF1, m-IF1 and m-FOF1.

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Authors and Affiliations

  1. Department of Biomedical Sciences and Technologies, University of Udine, p.le Kolbe 4, I-33100, Udine, Italy

    Valentina Giorgio & Elena Bisetto

  2. Department of Food Science, University of Udine, Via Sondrio 2/A, I-33100, Udine, Italy

    Giovanna Lippe

  3. Department of Life Sciences, University of Trieste, via L. Giorgieri 1, I-34127, Trieste, Italy

    Raffaella Franca & Sabina Passamonti

  4. Department of Biochemistry, University of Oxford, OX1 3QU, Oxford, England, UK

    David A. Harris

Authors
  1. Valentina Giorgio
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  2. Elena Bisetto
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  3. Raffaella Franca
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  4. David A. Harris
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  5. Sabina Passamonti
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  6. Giovanna Lippe
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Correspondence to Sabina Passamonti or Giovanna Lippe.

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Giorgio, V., Bisetto, E., Franca, R. et al. The ectopic FOF1 ATP synthase of rat liver is modulated in acute cholestasis by the inhibitor protein IF1 . J Bioenerg Biomembr 42, 117–123 (2010). https://doi.org/10.1007/s10863-010-9270-2

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  • DOI: https://doi.org/10.1007/s10863-010-9270-2

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