Abstract
This study validated the utilization of triacylglycerides (TAGs) by Pseudomonas mendocina CH50, a wild type strain, resulting in the production of novel mcl-PHAs with unique physical properties. A PHA yield of 58% dcw was obtained using 20 g/L of coconut oil. Chemical and structural characterisation confirmed that the mcl-PHA produced was a terpolymer comprising of three different repeating monomer units, 3-hydroxyoctanoate, 3-hydroxydecanoate and 3-hydroxydodecanoate or P(3HO-3HD-3HDD). Bearing in mind the potential of P(3HO-3HD-3HDD) in biomedical research, especially in neural tissue engineering, in vitro biocompatibility studies were carried out using NG108-15 (neuronal) cells. Cell viability data confirmed that P(3HO-3HD-3HDD) supported the attachment and proliferation of NG108-15 and was therefore confirmed to be biocompatible in nature and suitable for neural regeneration.
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Acknowledgements
The authors would like to thank the ReBioStent project – European Union’s Seventh Programme for research, technological development and demonstration under grant agreement number 604251, HyMedPoly project - Horizon 2020 under the grant agreement no 643050 and Neurimp project under grant agreement Grant Agreement No. 604450 for the funding provided to PB, BL, EM and RN. Finally, the authors would also like to thank Dr. Nicola Mordan from Eastman Dental Institute, University College London for her contribution in SEM imaging.
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Basnett, P., Marcello, E., Lukasiewicz, B. et al. Biosynthesis and characterization of a novel, biocompatible medium chain length polyhydroxyalkanoate by Pseudomonas mendocina CH50 using coconut oil as the carbon source. J Mater Sci: Mater Med 29, 179 (2018). https://doi.org/10.1007/s10856-018-6183-9
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DOI: https://doi.org/10.1007/s10856-018-6183-9