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Preparation of NGF encapsulated chitosan nanoparticles and its evaluation on neuronal differentiation potentiality of canine mesenchymal stem cells

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

Sustained and controlled release of neurotrophic factors in target tissue through nanomaterial based delivery system could be a better strategy for nerve tissue regeneration. The present study aims to prepare the nerve growth factor (NGF) encapsulated chitosan nanoparticles (NGF-CNPs) and its evaluation on neuronal differentiation potentiality of canine bone marrow derived mesenchymal stem cells (cBM-MSCs). The NGF-CNPs were prepared by ionotropic gelation method with tripolyphosphate (TPP) as an ionic cross-linking agent. Observations on physiochemical properties displayed the size of nanoparticles as 80–90 nm with positive zeta potential as well as an ionic interaction between NGF and nanoparticle. NGF loading efficiency was found to be 61% while its sustained release was observed by an in vitro release kinetics study. These nanoparticles were found to be cytocompatible to cBM-MSCs when supplemented at a concentration upto 4 mg/ml in culture media. The NGF-CNP supplemented culture media was able to transdifferentiate the preinduced cBM-MSCs into neurons in a better way than unbound NGF supplementation. Further, it was also noticed that NGF-CNPs were able to transdifferentiate cBM-MSCs without any chemical based preinduction. In conclusion, our findings propose that NGF-CNPs are capable of releasing bioactive NGF with the ability to transdifferentiate mesenchymal stem cells into neurons, suggesting its potential future application in nerve tissue regeneration.

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Acknowledgements

The authors are thankful to Director, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P., India for providing the facilities in carrying out this study.

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Authors and Affiliations

  1. Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, UP, 243122, India

    Bhabesh Mili, Kinsuk Das & Sadhan Bag

  2. Biochemistry and Food Science Section, ICAR-Indian Veterinary Research Institute, Izatnagar, UP, 243122, India

    Ajay Kumar

  3. Division of Surgery, ICAR-Indian Veterinary Research Institute, Izatnagar, UP, 243122, India

    A. C. Saxena

  4. Biophysics, Electron Microscopy and Instrumentation Section, ICAR-Indian Veterinary Research Institute, Izatnagar, UP, 243122, India

    Praveen Singh

  5. Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, UP, 243122, India

    Srikanta Ghosh

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  1. Bhabesh Mili
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Correspondence to Sadhan Bag.

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Mili, B., Das, K., Kumar, A. et al. Preparation of NGF encapsulated chitosan nanoparticles and its evaluation on neuronal differentiation potentiality of canine mesenchymal stem cells. J Mater Sci: Mater Med 29, 4 (2018). https://doi.org/10.1007/s10856-017-6008-2

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