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Porous bioactive scaffolds: characterization and biological performance in a model of tibial bone defect in rats

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Abstract

The aim of this study was to evaluate the effects of highly porous Biosilicate® scaffolds on bone healing in a tibial bone defect model in rats by means of histological evaluation (histopathological and immunohistochemistry analysis) of the bone callus and the systemic inflammatory response (immunoenzymatic assay). Eighty Wistar rats (12 weeks-old, weighing ±300 g) were randomly divided into 2 groups (n = 10 per experimental group, per time point): control group and Biosilicate® group (BG). Each group was euthanized 3, 7, 14 and 21 days post-surgery. Histological findings revealed a similar inflammatory response in both experimental groups, 3 and 7 days post-surgery. During the experimental periods (3–21 days post-surgery), it was observed that the biomaterial degradation, mainly in the periphery region, provided the development of the newly formed bone into the scaffolds. Immunohistochemistry analysis demonstrated that the Biosilicate® scaffolds stimulated cyclooxygenase-2, vascular endothelial growth factor and runt-related transcription factor 2 expression. Furthermore, in the immunoenzymatic assay, BG presented no difference in the level of tumor necrosis factor alpha in all experimental periods. Still, BG showed a higher level of interleukin 4 after 14 days post-implantation and a lower level of interleukin 10 in 21 days post-surgery. Our results demonstrated that Biosilicate® scaffolds can contribute for bone formation through a suitable architecture and by stimulating the synthesis of markers related to the bone repair.

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Acknowledgments

The authors thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) for their financial support.

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Authors and Affiliations

  1. Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil

    Hueliton Wilian Kido, Carla Roberta Tim & Nivaldo Antônio Parizotto

  2. Department of Physiological Sciences, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil

    Cynthia Aparecida de Castro

  3. Department of Materials Engineering, Vitreous Materials Laboratory (LaMaV), Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil

    Murilo Camuri Crovace, Ana Candida Martins Rodrigues, Edgar Dutra Zanotto & Oscar Peitl Filho

  4. Department of Morphology and Pathology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil

    Fernanda de Freitas Anibal

  5. Department of Biosciences, Federal University of São Paulo (UNIFESP), Ana Costa, 95, Santos, SP, Brazil

    Paulo Sérgio Bossini & Ana Claudia Muniz Rennó

Authors
  1. Hueliton Wilian Kido
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  2. Carla Roberta Tim
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  3. Paulo Sérgio Bossini
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  7. Ana Candida Martins Rodrigues
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  11. Ana Claudia Muniz Rennó
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Correspondence to Ana Claudia Muniz Rennó.

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Kido, H.W., Tim, C.R., Bossini, P.S. et al. Porous bioactive scaffolds: characterization and biological performance in a model of tibial bone defect in rats. J Mater Sci: Mater Med 26, 74 (2015). https://doi.org/10.1007/s10856-015-5411-9

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  • DOI: https://doi.org/10.1007/s10856-015-5411-9

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