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Accelerated endothelialization with a polymer-free sirolimus-eluting antibody-coated stent

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Abstract

Drug-eluting stents have shown an impressive reduction of in-stent restenosis for many years. However, stent thrombosis due to incomplete/late endothelialization has raised major safety concerns. To overcome these problems, we developed for the first time a polymer-free sirolimus-eluting antibody-coated stent (PFSEACS) by combining polymer free and endothelial progenitor cell-capture pro-healing approaches. In the first phase, the stents were prepared by loading sirolimus on the porous outer stent surface and directly fixing the anti-CD34 antibodies without any medium carriers on the blood contacting surface. The dose and elution of sirolimus, the amount and stability of anti-CD34 antibody immobilization, and the rate of CD34+ cell capture were evaluated. In the second phase, the stents were validated in an animal model of coronary arteries in pigs. The stent was observed to start collecting endothelial progenitor cells ~2 h after stent implantation and exhibited greatly enhanced endothelialization while maintaining an excellent anti-restenosis activity comparable to the polymer-free sirolimus-eluting stents. Overall, both in vitro and in vivo evaluations indicated that novel PFSEACSs exhibited facilitated endothelialization with excellent anti-restenosis activity and thus should merit further clinical studies.

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Acknowledgments

The present study was supported by Nature Science Foundation of China (NSFC #30770581 and 20971008) and Research Fund for the Doctoral Program of Higher Education of China.

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Authors and Affiliations

  1. State Key Laboratory of Natural and Biomimetic Drugs and Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, People’s Republic of China

    Zhanjiang Yu & Xiaoda Yang

  2. Department of Cardiology, Beijing Anzhen Hospital of the Capital University of Medical Sciences, Beijing, 100029, People’s Republic of China

    Huagang Zhu & Shuzheng Lü

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  1. Zhanjiang Yu
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  2. Huagang Zhu
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  3. Shuzheng Lü
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  4. Xiaoda Yang
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Correspondence to Xiaoda Yang.

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Yu, Z., Zhu, H., Lü, S. et al. Accelerated endothelialization with a polymer-free sirolimus-eluting antibody-coated stent. J Mater Sci: Mater Med 24, 2601–2609 (2013). https://doi.org/10.1007/s10856-013-5009-z

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  • DOI: https://doi.org/10.1007/s10856-013-5009-z

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