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Characterization of and host response to tyramine substituted-hyaluronan enriched fascia extracellular matrix

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An Erratum to this article was published on 18 June 2011

Abstract

Naturally-occurring biomaterial scaffolds derived from extracellular matrix (ECM) have been previously investigated for soft tissue repair. We propose to enrich fascia ECM with high molecular weight tyramine substituted-hyaluronan (TS-HA) to modulate inflammation associated with implantation and enhance fibroblast infiltration. As critical determinants of constructive remodeling, the host inflammatory response and macrophage polarization to TS-HA enriched fascia were characterized in a rat abdominal wall model. TS-HA treated fascia with cross-linking had a similar lymphocyte (P = 0.11) and plasma cell (P = 0.13) densities, greater macrophage (P = 0.001) and giant cell (P < 0.0001) densities, and a lower density of fibroblast-like cells (P < 0.0001) than water treated controls. Treated fascia, with or without cross-linking, exhibited a predominantly M2 pro-remodeling macrophage profile similar to water controls (P = 0.82), which is suggestive of constructive tissue remodeling. Our findings demonstrated that HA augmentation can alter the host response to an ECM, but the appropriate concentration and molecular weight needed to minimize chronic inflammation within the scaffold remains to be determined.

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Acknowledgments

This study was financially supported by the National Institutes of Health (NIAMS Grant # R01AR056633, F31AR057305, T32AR50959) and the Ohio Third Frontier (Grant # BRTT05-30). The authors would like to acknowledge the Musculoskeletal Transplant Foundation for their fascia donation, Lifecore Biomedical for their TS-HA donation, and Michael Braun (Biomedical Engineering Histology Core, Cleveland Clinic) for the histologic preparation of the H&E stained sections.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, ND20, Cleveland, OH, 44195, USA

    LiKang Chin, Anthony Calabro & Kathleen A. Derwin

  2. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA

    LiKang Chin

  3. Department of Anatomic Pathology, Cleveland Clinic, Cleveland, OH, 44195, USA

    E. Rene Rodriguez & Carmela D. Tan

  4. Department of Quantitative Health Sciences, Cleveland Clinic, Cleveland, OH, 44195, USA

    Esteban Walker

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  1. LiKang Chin
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  2. Anthony Calabro
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Correspondence to Kathleen A. Derwin.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s10856-011-4373-9

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Chin, L., Calabro, A., Rodriguez, E.R. et al. Characterization of and host response to tyramine substituted-hyaluronan enriched fascia extracellular matrix. J Mater Sci: Mater Med 22, 1465–1477 (2011). https://doi.org/10.1007/s10856-011-4325-4

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