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Characterization and biological application of YAG:Ce3+ nanophosphor modified with mercaptopropyl trimethoxy silane

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Abstract

We focus on the inorganic nanophosphor of YAG:Ce3+ nanoparticles as an alternative fluorescent probe instead of organic dyes for biological application. YAG:Ce3+ nanoparticles have the green emission at 530 nm under the excitation of blue light at 450 nm. Conventional biochemical equipments for organic dyes can be used for YAG:Ce3+ nanoparticles. SH groups were introduced by surface modification of YAG:Ce3+ nanoparticles with 3-mercaptopropyl trimethoxy silane, and were characterized by X-ray fluorescence analysis, FT-IR, and Ellman method. We demonstrated tagging avidin-immobilized agarose gel beads with biotinylated-YAG:Ce3+ nanoparticles, and tagging rabbit IgG-immobilized agarose gel beads with antirabbit IgG-immobilized YAG:Ce3+ nanoparticles.

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Abbreviations

YAG:Ce3+ :

Yttrium aluminum garnet doped with Ce3+

APTMS:

3-Aminopropyl trimethoxy silane

MPTMS:

3-Mercaptopropyl trimethoxy silane

1,4-BD:

1,4-Butanediol

EMCS:

n-(6-Maleimidocaproyloxy) sulfosuccinimide

DTNB:

5,5′-Dithio-bis-2-nitrobenzoic acid

TNB:

5-Thio-2-nitrobenzoic acid

XRF:

X-ray fluorescence analysis

UV–vis:

Ultraviolet–visible

PL:

Photoluminescence

PLE:

Photoluminescence excitation

DLS:

Dynamic light scattering method

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Authors and Affiliations

  1. Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan

    Atsushi Tsukamoto & Tetsuhiko Isobe

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  1. Atsushi Tsukamoto
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  2. Tetsuhiko Isobe
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Correspondence to Tetsuhiko Isobe.

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Tsukamoto, A., Isobe, T. Characterization and biological application of YAG:Ce3+ nanophosphor modified with mercaptopropyl trimethoxy silane. J Mater Sci 44, 1344–1350 (2009). https://doi.org/10.1007/s10853-008-3012-4

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  • DOI: https://doi.org/10.1007/s10853-008-3012-4

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